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Yasuaki Kawarasaki
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PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets
T Kojima, Y Takei, M Ohtsuka, Y Kawarasaki, T Yamane, H Nakano
Nucleic acids research 33 (17), e150-e150, 2005
2232005
Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans
K Ito, S Ito, T Shimamura, S Weyand, Y Kawarasaki, T Misaka, K Abe, ...
Journal of molecular biology 408 (2), 177-186, 2011
1722011
Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides
D Tullman-Ercek, MP DeLisa, Y Kawarasaki, P Iranpour, B Ribnicky, ...
Journal of Biological Chemistry 282 (11), 8309-8316, 2007
1722007
Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor
VTT Lan, K Ito, M Ohno, T Motoyama, S Ito, Y Kawarasaki
Food Chemistry 175, 66-73, 2015
1192015
Enhanced crossover SCRATCHY: construction and high‐throughput screening of a combinatorial library containing multiple non‐homologous crossovers
Y Kawarasaki, KE Griswold, JD Stevenson, T Selzer, SJ Benkovic, ...
Nucleic acids research 31 (21), e126-e126, 2003
902003
High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression
S Rungpragayphan, Y Kawarasaki, T Imaeda, K Kohda, H Nakano, ...
Journal of molecular biology 318 (2), 395-405, 2002
792002
Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library
K Ito, A Hikida, S Kawai, VTT Lan, T Motoyama, S Kitagawa, Y Yoshikawa, ...
Nature communications 4 (1), 2502, 2013
772013
Evolution of highly active enzymes by homology-independent recombination
KE Griswold, Y Kawarasaki, N Ghoneim, SJ Benkovic, BL Iverson, ...
Proceedings of the National Academy of Sciences 102 (29), 10082-10087, 2005
682005
A long-lived batch reaction system of cell-free protein synthesis
Y Kawarasaki, T Kawai, H Nakano, T Yamane
Analytical biochemistry 226 (2), 320-324, 1995
651995
A periplasmic fluorescent reporter protein and its application in high-throughput membrane protein topology analysis
KJ Jeong, Y Kawarasaki, J Gam, BR Harvey, BL Iverson, G Georgiou
Journal of molecular biology 341 (4), 901-909, 2004
602004
An increased rate of cell-free protein synthesis by condensing wheat-germ extract with ultrafiltration membranes
H Nakano, T Tanaka, Y Kawarasaki, T Yamane
Bioscience, biotechnology, and biochemistry 58 (4), 631-634, 1994
531994
Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae
A Mori, S Hara, T Sugahara, T Kojima, Y Iwasaki, Y Kawarasaki, T Sahara, ...
Journal of bioscience and bioengineering 120 (5), 518-525, 2015
482015
Prolonged cell-free protein synthesis in a batch system using wheat germ extract
Y Kawarasaki, H Nakano, T Yamane
Bioscience, biotechnology, and biochemistry 58 (10), 1911-1913, 1994
421994
Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV
VTT Lan, K Ito, S Ito, Y Kawarasaki
Peptides 54, 166-170, 2014
382014
Highly productive cell-free protein synthesis system using condensed wheat-germ extract
H Nakano, T Tanaka, Y Kawarasaki, T Yamane
Journal of biotechnology 46 (3), 275-282, 1996
381996
Chimeric gene library construction by a simple and highly versatile method using recombination‐dependent exponential amplification
A Ikeuchi, Y Kawarasaki, T Shinbata, T Yamane
Biotechnology progress 19 (5), 1460-1467, 2003
302003
Purification and some properties of wheat germ acid phosphatases
Y Kawarasaki, H Nakano, T Yamane
Plant Science 119 (1-2), 67-77, 1996
301996
Systematic analysis of a dipeptide library for inhibitor development using human dipeptidyl peptidase IV produced by a Saccharomyces cerevisiae expression system
A Hikida, K Ito, T Motoyama, R Kato, Y Kawarasaki
Biochemical and biophysical research communications 430 (4), 1217-1222, 2013
282013
Regulation of RraA, a protein inhibitor of RNase E-mediated RNA decay
M Zhao, L Zhou, Y Kawarasaki, G Georgiou
Journal of bacteriology 188 (9), 3257-3263, 2006
282006
Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class genes
D Chen, Y Kawarasaki, H Nakano, T Yamane
Journal of bioscience and bioengineering 95 (6), 594-600, 2003
282003
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Articles 1–20