- ►alphamedpress.org H Segev, B Fishman, A Ziskind, M Shulman, J Itskovitz- … - Stem Cells, 2004 - AlphaMed Press The protocol consisted of several steps. Embryoid bodies were first cultured and plated in
insulin-transferrin-selenium-fibronectin medium, followed by medium supplemented with N2,
B27, and basic fibroblast growth factor (bFGF). Next, the glucose concentration in the ... Cited by 216 - Related articles - BL Direct - All 7 versions
- ►alphamedpress.org HT Ku, N Zhang, A Kubo, R O'Connor, M Mao, G Keller, … - Stem Cells, 2004 - AlphaMed Press A panel of genetic markers was used to assess the in vitro commitment of murine embryonic
stem (ES) cells toward the endoderm-derived pancreas and to distinguish insulin-expressing
cells of this lineage from other lineages such as neuron, liver, and yolk sac. There are two ... Cited by 197 - Related articles - All 7 versions
- ►diabetesjournals.org S Miyazaki, E Yamato, J Miyazaki - Diabetes, 2004 - Am Diabetes Assoc Embryonic stem (ES) cells can differentiate into many cell types. Recent reports have shown
that ES cells can differentiate into insulin-producing cells. However, the differentiation is not efficient
enough to produce insulin-secreting cells for future therapeutic use. Pdx-1, a ... Cited by 155 - Related articles - BL Direct - All 4 versions
M Hansson, A Tonning, U Frandsen, A Petri, J … - Diabetes, 2004 - Am Diabetes Assoc Several recent reports claim the generation of insulin-producing cells from embryonic stem cells
via the differentiation of progenitors that express nestin. Here, we investigate further the properties
of these insulin-containing cells. We find that although differentiated cells contain ... Cited by 139 - Related articles - BL Direct - All 4 versions
- ►neiltheise.com [PDF] MA Hussain, ND Theise - The Lancet, 2004 - Elsevier Curative therapy for diabetes mellitus mainly implies replacement of functional insulin-producing
pancreatic β cells, with pancreas or islet-cell transplants. However, shortage of donor organs
spurs research into alternative means of generating β cells from islet expansion, ... Cited by 83 - Related articles - All 8 versions
- ►diabetesjournals.org GKC Brolén, N Heins, J Edsbagge, H Semb - Diabetes, 2005 - Am Diabetes Assoc The recent success in restoring normoglycemia in type 1 diabetes by islet cell transplantation
indicates that cell replacement therapy of this severe disease is achievable. However, the severe
lack of donor islets has increased the demand for alternative sources of β-cells, such as ... Cited by 81 - Related articles - BL Direct - All 6 versions
M Narushima, N Kobayashi, T Okitsu, Y … - Nature …, 2005 - journals2005.pasteur.ac.ir A human pancreatic b-cell line that is functionally equivalent to primary b-cells has not been
available. We established a reversibly immortalized human b-cell clone (NAKT-15) by transfection
of primary human b-cells with a retroviral vector containing simian virus 40 large ... Cited by 71 - Related articles - BL Direct - All 6 versions
Y Hori, X Gu, X Xie, SK Kim - PLoS Medicine, 2005 - medicine.plosjournals.org Here we show that brain-derived human neural progenitor cells, exposed to a series of signals
that regulate in vivo pancreatic islet development, form clusters of glucose-responsive
insulin-producing cells (IPCs). During in vitro differentiation of neural progenitor cells with ... Cited by 67 - Related articles - Cached - BL Direct - All 13 versions
P Blyszczuk, C Asbrand, A Rozzo, G Kania, L St-Onge, … - Int J Dev Biol, 2004 - ijdb.ehu.es ABSTRACT We present a new strategy for the differentiation of embryonic stem (ES) cells into
insulin-producing cells via a multi-step process without selection and induction of nestin-positive
cells. During ES cell differentiation, transcript levels of genes characteristic of early and ... Cited by 66 - Related articles - View as HTML - All 6 versions
N Ende, R Chen, AS Reddi - Biochemical and Biophysical Research …, 2004 - Elsevier Several studies have shown that transplantation of embryonic stem cells into diabetic animals
either improved or normalized blood glucose levels. In this study, we examined the dose-dependent
effect of early (prediabetic stage) intravenous administration of human umbilical cord ... Cited by 60 - Related articles - All 5 versions
