SJ Hunter, AC Boyd, FPM O'Harte, AM McKillop, MI … - Diabetes, 2003 - Am Diabetes Assoc The presence and biological significance of circulating glycated insulin has been evaluated by
high-pressure liquid chromatography (HPLC), electrospray ionization mass spectrometry
(ESI-MS), radioimmunoassay (RIA), receptor binding, and hyperinsulinemic-euglycemic ... Cited by 20 - Related articles - BL Direct - All 4 versions
AM McKillop, MH Mooney, P Harriott, PR Flatt, FPM … - … and Biophysical Research …, 2001 - Elsevier Glycated insulin was evaluated in plasma and biological tissues of diabetic animal models by
immunocytochemistry (ICC) and a novel radioimmunoassay. Glycated insulin circulated at 0.10
± 0.04 ng/ml and 2.20 ± 0.14 ng/ml in lean and diabetic obese (ob/ob) mice, ... Cited by 11 - Related articles - BL Direct - All 5 versions
AC Boyd, YHA Abdel-Wahab, AM McKillop, H Mcnulty, … - BBA-General Subjects, 2000 - Elsevier Previous studies have shown that glycated insulin is secreted from pancreatic β-cells under conditions
of hyperglycaemia. This study has investigated the effects of monoglycated insulin on plasma
glucose homeostasis and in vitro cellular glucose transport and metabolism by isolated ... Cited by 12 - Related articles - All 4 versions
A Lapolla, P Tessari, T Poli, A Valerio, E Duner, E Iori, … - Diabetes, 1988 - Am Diabetes Assoc We evaluated the in vivo biological activity of in vitro extensively glycosylated insulin (GI) with
the euglycemic-hyperinsulinemic glucose-clamp technique in postabsorptive nondiabetic
subjects. Insulin-mediated glucose disposal was approximately 30% lower (P less than ... Cited by 11 - Related articles - All 5 versions
YHA Abdel-Wahab, FPM O'Harte, AC Boyd, CR Barnett … - Acta Diabetologica, 1997 - Springer Abstract Bovine insulin was glycated by in vitro incu- bation with 20–220 mM D -glucose for
1–48 h. The per- centage of glycation was dependent on time, glucose con- centration, temperature
and pH, attaining values up to 28%. Glucose-lowering activities of glycated and control ... Cited by 18 - Related articles - BL Direct - All 3 versions
- ►endocrinology-journals.org [PDF] AM McKillop, JT McCluskey, AC Boyd, MH … - Journal of …, 2000 - Soc Endocrinology Previous studies have shown that glycation of insulin occurs in pancreatic -cells under conditions
of hypergly- caemia and that the site of glycation is the N-terminal Phe1 of the insulin
B-chain. To enable evaluation of glycated insulin in diabetes, specific antibodies were ... Cited by 18 - Related articles - BL Direct - All 4 versions
YH Abdel-Wahab, FP O'harte, H Ratcliff, NH … - Diabetes, 1996 - Am Diabetes Assoc The glycation of immunoreactive insulin (IRI) was assessed in extracts of pancreas and islets
from control and hyperglycemic animal models. Glycated and nonglycated IRI were separated
by affinity chromatography and quantified by radioimmunoassay. Hydrocortisone-treated ... Cited by 45 - Related articles - BL Direct - All 5 versions
FP O'Harte, P Højrup, CR Barnett, PR Flatt - Peptides, 1996 - ncbi.nlm.nih.gov This study evaluates the nature of glycated human insulin formed following exposure to hyperglycemic
conditions in vitro. Glycated insulin was purified by RP-HPLC and its molecular mass (5971.3
Da) determined by plasma desorption mass spectrometry (MS). The difference in mass ( ... Cited by 24 - Related articles - All 3 versions
NH McClenaghan - Nutrition research reviews, 2005 - Cambridge Univ Press Insulin resistance underlies type 2 diabetes, CVD and the metabolic syndrome, driven by changes
in diet, lifestyle, energy over-consumption and obesity. Nutritional recommendations for insulin
resistance remain an area of controversy, particularly the quantity and types of dietary ... Cited by 8 - Related articles - BL Direct - All 6 versions
JR Lindsay, AM McKillop, MH Mooney, FPM O'Harte, … - Diabetologia, 2003 - Springer Aims/hypothesis. Glycation of insulin, resulting in im- paired bioactivity, has been shown within
pancreatic beta cells. We have used a novel and specific radioim- munoassay to detect glycated
insulin in plasma of Type 2 diabetic subjects. Methods. Blood samples were collected ... Cited by 10 - Related articles - BL Direct - All 4 versions
