M Hansson, A Tonning, U Frandsen, A Petri, J … - Diabetes, 2004 - Am Diabetes Assoc Several recent reports claim the generation of insulin-producing cells from embryonic stem cells
via the differentiation of progenitors that express nestin. Here, we investigate further the properties
of these insulin-containing cells. We find that although differentiated cells contain ... Cited by 139 - Related articles - BL Direct - All 4 versions
S Sipione, A Eshpeter, JG Lyon, GS Korbutt, RC … - Diabetologia, 2004 - Springer Aim/hypothesis. Embryonic stem (ES) cells have been proposed as a potential source of tissue
for transplan- tation for the treatment of Type 1 diabetes. However, studies showing differentiation
of beta cells from ES cells are controversial. The aim of this study was to characterise the ... Cited by 123 - Related articles - BL Direct - All 6 versions
- ►colorado.edu [PDF] J Rajagopal, WJ Anderson, S Kume, OI Martinez, DA … - Science, 2003 - sciencemag.org Despite antibody staining, we did not detect insulin 1 mRNA by reverse transcription-polymerase
chain reaction (RT-PCR) and insulin 2 mRNA detection was weak. Multiple primers used during
all five stages of the protocol (1) confirmed these results. RT-PCR controls detected ... Cited by 359 - Related articles - All 5 versions
- ►imim.cat [PDF] T Leon-Quinto, J Jones, A Skoudy, M Burcin, B Soria - Diabetologia, 2004 - Springer Aims/hypothesis. We recently demonstrated that insu- lin-producing cells derived from embryonic
stem cells normalise hyperglycaemia in transplanted diabetic mice. The differentiation and selection
procedure, however, was successful in less than 5% of the assays performed. Thus, to ... Cited by 119 - Related articles - BL Direct - All 7 versions
Y Moritoh, E Yamato, Y Yasui, S Miyazaki, J Miyazaki - Diabetes, 2003 - Am Diabetes Assoc Embryonic stem (ES) cells can differentiate into many cell types and are expected to be useful
for tissue engineering. Recent reports have shown that ES cells can differentiate into
insulin-producing cells in response to the transient expression of the pdx-1 gene, after the ... Cited by 123 - Related articles - BL Direct - All 4 versions
P Blyszczuk, C Asbrand, A Rozzo, G Kania, L St-Onge, … - Int J Dev Biol, 2004 - ijdb.ehu.es ABSTRACT We present a new strategy for the differentiation of embryonic stem (ES) cells into
insulin-producing cells via a multi-step process without selection and induction of nestin-positive
cells. During ES cell differentiation, transcript levels of genes characteristic of early and ... Cited by 66 - Related articles - View as HTML - All 6 versions
- ►alphamedpress.org H Segev, B Fishman, A Ziskind, M Shulman, J Itskovitz- … - Stem Cells, 2004 - AlphaMed Press The protocol consisted of several steps. Embryoid bodies were first cultured and plated in
insulin-transferrin-selenium-fibronectin medium, followed by medium supplemented with N2,
B27, and basic fibroblast growth factor (bFGF). Next, the glucose concentration in the ... Cited by 216 - Related articles - BL Direct - All 7 versions
Y Hori, IC Rulifson, BC Tsai, JJ Heit, JD … - Proceedings of the …, 2002 - National Acad Sciences The use of embryonic stem cells for cell-replacement therapy in diseases like diabetes mellitus
requires methods to control the development of multipotent cells. We report that treatment of
mouse embryonic stem cells with inhibitors of phosphoinositide 3-kinase, an essential ... Cited by 343 - Related articles - BL Direct - All 10 versions
- ►diabetesjournals.org S Miyazaki, E Yamato, J Miyazaki - Diabetes, 2004 - Am Diabetes Assoc Embryonic stem (ES) cells can differentiate into many cell types. Recent reports have shown
that ES cells can differentiate into insulin-producing cells. However, the differentiation is not efficient
enough to produce insulin-secreting cells for future therapeutic use. Pdx-1, a ... Cited by 155 - Related articles - BL Direct - All 4 versions
P Blyszczuk, J Czyz, G Kania, M Wagner, … - Proceedings of the …, 2003 - National Acad Sciences Mouse embryonic stem (ES) cells differentiate into cells of all three primary germ layers including
endodermal cells that produce insulin in vitro. We show that constitutive expression of Pax4
(Pax4 + ), and to a lesser extent Pdx1 (Pdx1 + ), affects the differentiation of ES cells and ... Cited by 327 - Related articles - BL Direct - All 11 versions
