Authors
Hamidreza Ebrahimiyan, Farhad Gharibdoost, Saeed Aslani, Hoda Kavosi, Faraneh Farsad, Ahmadreza Jamshidi, Mahdi Mahmoudi
Publication date
2020/9/2
Journal
Modern Rheumatology
Volume
30
Issue
5
Pages
862-869
Publisher
Oxford University Press
Description
Background: Survivin is an important anti-apoptotic protein and is involved in increasing auto-reactivity during the autoimmune diseases like systemic sclerosis (SSc).
Aims: In the current study, we investigate the expression level of total survivin (survivin-TS) and its three important variants alongside with evaluation of the expression level of important microRNAs (miRNAs) that are involved in survivin expression regulation.
Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from 50 healthy controls, 25 diffuse cutaneous SSc (DcSSc), and 25 limited cutaneous SSc (LcSSc) patients. RNA was extracted and single-strand cDNA was synthesized. Quantitative real-time PCR was used to evaluate the expression level of survivin-TS and its variants as well the miRNAs.
Results: Overexpression of survivin-2B and downregulation of survivin wild-type (survivin-WT …
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Scholar articles
H Ebrahimiyan, F Gharibdoost, S Aslani, H Kavosi… - Modern Rheumatology, 2020