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In this investigation, 5 ml blood from sixty four Iranian patients with confirmed diagnosis of RAS 5 was collected in the EDTA tubes. DNA was extracted using a phenol-chloroform method. This project was approved by Ethics Committee of Tehran University of Medical Sciences. Written informed consent was obtained from all subjects before sampling. IL12 gene typing was performed by Polymerase Chain Reaction with Sequence-Specific Primers (PCR-SSP) assay (PCR-SSP kit, Heidelberg University, Heidelberg, Germany), similar to what explained before 6. The allele and genotype frequencies of IL12 (A-1188 C) were investigated. Allele frequencies were estimated by direct gene counting. The results were compared to the number of alleles and genotypes in 140 healthy controls from the same region. Chi-square test was used to compare frequencies of alleles, genotypes and haplotypes between patients and …