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The aim of this study was to evaluate the psychometric properties of tools" Team-Based Learning Student Assessment Tool", Classroom Engagement Survey (CES) and to assess the reaction of learners toward TBL sessions at Tehran University of Medical Sciences. This descriptive study was done in 2013. The first step was to assess the reliability and validity of the tools. TBL-SAI questionnaire include 39 items, and CES consists 8 items. The validity was assessed through Delphi rounds by experts and reliability, through internal consistency and Test-Retest approach. Then, the reaction of medical students (N= 78) was assessed concerning the aspects of team-based learning sessions through TBL-SAI and CES. The data were analyzed through descriptive tests. Our results have study confirmed the TBL-SAI and CEA validity. The tools’ reliability was approved through: TBL-SAI Cronbach's alpha= 0.79, CES Cronbach's alpha= 0.71 and TBL-SAI ICC= 0.82, CES ICC= 0.75. The result of the second phase showed the TBL_SAI scores of participation were appropriate concerning TBL session (12.89±159.60). According to confirmed validity of tools, these can be used in researches related to team-based learning in Iran. It could facilitate assessing the learners’ reaction of team-based learning studies at Iranian medical science universities. In the present study, the reaction of students who participate in TBL sessions had been positive and their participation, satisfaction, and accountability had been improved.