Authors
Katayoon Bidad, Eisa Salehi, Ahmadreza Jamshidi, Ali Akbar Saboor-Yaraghi, Mona Oraei, Alipasha Meysamie, Mahdi Mahmoudi, Mohammad Hossein Nicknam
Publication date
2013/4/1
Journal
The Journal of rheumatology
Volume
40
Issue
4
Pages
476-483
Publisher
The Journal of Rheumatology
Description
Objective
We compared Th17 and T regulatory cells in patients with ankylosing spondylitis (AS) and in healthy controls. The effect of all-transretinoic acid (ATRA) was studied on cultured CD4+ T cells of patients with AS compared to controls.
Methods
Eighteen patients with AS and 18 age- and sex-matched healthy controls were included. CD4+ T cells were separated and cultured in conditions of anti-CD3 and anti-CD28 stimulation with and without ATRA. Intracellular and secreted cytokines, transcription factors, and gene expression were evaluated after 72 h.
Results
The frequency of CD4+IL-17+ T cells was significantly higher in patients with AS compared to controls, and ATRA could significantly decrease it. The frequency of forkhead box protein 3 (FOXP3)+ retinoic acid-related orphan receptor γt (RORγt) negative T-bet negative CD4+ cells was significantly lower in cases compared to controls. Intracellular …
Scholar articles
K Bidad, E Salehi, A Jamshidi, AA Saboor-Yaraghi… - The Journal of rheumatology, 2013