Authors
Ramazan Rezaei, Mahdi Mahmoudi, Farhad Gharibdoost, Hoda Kavosi, Navid Dashti, Vahideh Imeni, Ahmadreza Jamshidi, Saeed Aslani, Shayan Mostafaei, Mohammad Vodjgani
Publication date
2017/10
Journal
International journal of rheumatic diseases
Volume
20
Issue
10
Pages
1551-1561
Description
Objective
The aim of the current study was to evaluate if methylation status of CpG sites of interferon regulatory factor 7 (IRF7) promoter in peripheral blood mononuclear cells (PBMCs) of systemic sclerosis (SSc) patients is involved in pathogenesis of the disease.
Methods
PBMCs were isolated from whole blood of 50 SSc patients and 30 controls. After the extraction of total RNA and DNA contents from PBMCs, complementary DNA (cDNA) was synthesized. Afterwards, quantitative analysis of IRF7 messenger RNA (mRNA) was conducted by real‐time polymerase chain reaction (PCR). To evaluate the methylation status of the promoter region of IRF7 gene, PCR products of bisulfite‐treated DNA from SSc patients and controls were sequenced.
Results
The mRNA expression of IRF7 in PBMCs from patients compared with controls was significantly upregulated. While limited cutaneous SSc patients expressed the …
Scholar articles
R Rezaei, M Mahmoudi, F Gharibdoost, H Kavosi… - International journal of rheumatic diseases, 2017