Authors
Maryam Akhtari, Mahdi Vojdanian, Ali Javinani, Amir Ashraf-Ganjouei, Ahmadreza Jamshidi, Mahdi Mahmoudi
Publication date
2020/4/1
Journal
Cytokine
Volume
128
Pages
154997
Publisher
Academic Press
Description
Background
Ankylosing spondylitis (AS) is an auto-inflammatory debilitating disorder with a complex pathogenesis. The adenosinergic pathway is an immunologic regulating pathway with a potential role in AS pathophysiology. In the present study, we have aimed to investigate the influence of A2A adenosine receptor (A2AAR) activation on tumor necrosis factor-α (TNF-α) and interleukin-23 (IL-23) expression and secretion by monocyte-generated macrophages of AS patients.
Methods
Whole-blood separated monocytes were extracted from 14 AS patients and 14 healthy controls. Macrophages were differentiated by macrophage colony-stimulating factor (M-CSF), and surface markers were confirmed by flow cytometer. Cells were treated with CGS-21680 as a known agonist of A2AAR. Analysis of ADORA2A, TNFA, and IL23A gene expression was performed by SYBR green real-time PCR. The concentration of …
Total citations
Scholar articles
M Akhtari, M Vojdanian, A Javinani, A Ashraf-Ganjouei… - Cytokine, 2020