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Tomás Aparicio Pérez
Tomás Aparicio Pérez
Post-doctoral Researcher (CNB-CSIC, Spain
Verified email at cnb.csic.es
Title
Cited by
Cited by
Year
Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression
E Martínez-García, PI Nikel, T Aparicio, V de Lorenzo
Microbial cell factories 13, 1-15, 2014
2452014
SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities
E Martínez-García, T Aparicio, A Goñi-Moreno, S Fraile, V de Lorenzo
Nucleic acids research 43 (D1), D1183-D1189, 2015
2242015
CRISPR/Cas9‐Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida
T Aparicio, V de Lorenzo, E Martínez‐García
Biotechnology journal 13 (5), 1700161, 2018
1382018
New transposon tools tailored for metabolic engineering of Gram-negative microbial cell factories
E Martínez-García, T Aparicio, V de Lorenzo, PI Nikel
Frontiers in bioengineering and biotechnology 2, 46, 2014
1192014
Molecular characterization of the safracin biosynthetic pathway from Pseudomonas fluorescens A2‐2: designing new cytotoxic compounds
A Velasco, P Acebo, A Gomez, C Schleissner, P Rodríguez, T Aparicio, ...
Molecular microbiology 56 (1), 144-154, 2005
992005
The Ssr protein (T1E_1405) from Pseudomonas putida DOT‐T1E enables oligonucleotide‐based recombineering in platform strain P. putida EM42
T Aparicio, SI Jensen, AT Nielsen, V de Lorenzo, E Martínez‐García
Biotechnology journal 11 (10), 1309-1319, 2016
702016
A standardized workflow for surveying recombinases expands bacterial genome‐editing capabilities
DE Ricaurte, E Martínez‐García, A Nyerges, C Pal, V de Lorenzo, ...
Microbial Biotechnology 11 (1), 176-188, 2018
392018
CRISPR/Cas9‐enhanced ssDNA recombineering for Pseudomonas putida
T Aparicio, V de Lorenzo, E Martínez‐García
Microbial Biotechnology 12 (5), 1076-1089, 2019
362019
High-efficiency multi-site genomic editing of Pseudomonas putida through thermoinducible ssDNA recombineering
T Aparicio, A Nyerges, E Martínez-García, V de Lorenzo
Iscience 23 (3), 2020
352020
SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes
E Martínez-García, S Fraile, E Algar, T Aparicio, E Velázquez, B Calles, ...
Nucleic Acids Research 51 (D1), D1558-D1567, 2023
342023
Engineering Gram-negative microbial cell factories using transposon vectors
E Martínez-García, T Aparicio, V de Lorenzo, PI Nikel
In Vitro Mutagenesis: Methods and Protocols, 273-293, 2017
302017
Improved Thermotolerance of Genome‐Reduced Pseudomonas putida EM42 Enables Effective Functioning of the PL/cI857 System
T Aparicio, V de Lorenzo, E Martínez‐García
Biotechnology Journal 14 (1), 1800483, 2019
272019
Broadening the SEVA plasmid repertoire to facilitate genomic editing of Gram-negative bacteria
T Aparicio, V de Lorenzo, E Martínez-García
Hydrocarbon and lipid microbiology protocols: genetic, genomic and system …, 2017
262017
Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF gene
T Aparicio, A Nyerges, I Nagy, C Pal, E Martínez‐García, V de Lorenzo
Environmental microbiology 22 (1), 45-58, 2020
212020
Multifunctional SEVA shuttle vectors for actinomycetes and Gram‐negative bacteria
C García‐Gutiérrez, T Aparicio, L Torres‐Sánchez, E Martínez‐García, ...
Microbiologyopen 9 (6), 1135-1149, 2020
132020
A broad host range plasmid-based roadmap for ssDNA-based recombineering in Gram-negative bacteria
T Aparicio, V de Lorenzo, E Martínez-García
Horizontal Gene Transfer: Methods and Protocols, 383-398, 2020
102020
An automated DIY framework for experimental evolution of Pseudomonas putida
DR Espeso, P Dvořák, T Aparicio, V de Lorenzo
Microbial Biotechnology 14 (6), 2679-2685, 2021
82021
Functional mapping of PHF6 complexes in chromatin remodeling, replication dynamics, and DNA repair
S Alvarez, AC da Silva Almeida, R Albero, M Biswas, A Barreto-Galvez, ...
Blood, The Journal of the American Society of Hematology 139 (23), 3418-3429, 2022
72022
Refactoring the conjugation machinery of promiscuous plasmid RP4 into a device for conversion of gram-negative isolates to Hfr strains
J Silbert, V Lorenzo, T Aparicio
ACS Synthetic Biology 10 (4), 690-697, 2021
72021
pT3. 2I, the smallest plasmid of Thiobacillus T3. 2
T Aparicio, P Lorenzo, J Perera
Plasmid 44 (1), 1-11, 2000
72000
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