A review of lipidation in the development of advanced protein and peptide therapeutics R Menacho-Melgar, JS Decker, JN Hennigan, MD Lynch Journal of Controlled Release 295, 1-12, 2019 | 89 | 2019 |
Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity EA Moreb, B Hoover, A Yaseen, N Valyasevi, Z Roecker, ... ACS synthetic biology 6 (12), 2209-2218, 2017 | 48 | 2017 |
Scalable, two‐stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion R Menacho‐Melgar, Z Ye, EA Moreb, T Yang, JP Efromson, JS Decker, ... Biotechnology and Bioengineering, 2020 | 38 | 2020 |
Low-Cost, Large-Scale Production of the Anti-viral Lectin Griffithsin JS Decker, R Menacho-Melgar, MD Lynch Frontiers in bioengineering and biotechnology 8, 1020, 2020 | 30 | 2020 |
Improved, two‐stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease R Menacho‐Melgar, EA Moreb, JP Efromson, T Yang, JN Hennigan, ... Biotechnology and Bioengineering, 2020 | 29 | 2020 |
Media Robustness and Scalability of Phosphate Regulated Promoters Useful for Two-Stage Autoinduction in E. coli EA Moreb, Z Ye, JP Efromson, JN Hennigan, R Menacho-Melgar, ... ACS Synthetic Biology, 2020 | 28 | 2020 |
Escherichia coli Cas1/2 Endonuclease Complex Modifies Self-Targeting CRISPR/Cascade Spacers Reducing Silencing Guide Stability Z Ye, EA Moreb, S Li, J Lebeau, R Menacho-Melgar, M Munson, ... ACS Synthetic Biology 10 (1), 29-37, 2020 | 8 | 2020 |
Optimization of phosphate-limited autoinduction broth for two-stage heterologous protein expression in Escherichia coli R Menacho-Melgar, JN Hennigan, MD Lynch Biotechniques 71 (4), 566-572, 2021 | 7 | 2021 |
Instant Taq: Rapid Autoinducible Expression and Chromatography-free Purification of Taq polymerase R Menacho-Melgar, T Yang, MD Lynch bioRxiv, 2021 | 3 | 2021 |
Media robustness and scalability of phosphate regulated promoters useful for two-stage autoinduction in E. coli. ACS Synthetic Biology, 9, 1483–1486 EA Moreb, Z Ye, JP Efromson, JN Hennigan, R Menacho-Melgar, ... | 3 | 2020 |
Integrated autolysis, DNA hydrolysis and precipitation enables an improved bioprocess for Q-Griffithsin, a broad-spectrum antiviral and clinical-stage anti-COVID-19 candidate JS Decker, R Menacho-Melgar, MD Lynch Biochemical Engineering Journal 181, 108403, 2022 | 2 | 2022 |
Measuring Oligonucleotide Hydrolysis in Cellular Lysates via Viscosity Measurements R Menacho-Melgar, MD Lynch Bio-protocol 12 (2), e4304-e4304, 2022 | 1 | 2022 |
Optimization of Phosphate Limited Autoinduction Broth for 2-Stage Heterologous Protein Expression in E. coli R Menacho-Melgar, JN Hennigan, MD Lynch bioRxiv, 2021 | 1 | 2021 |
The E. coli Cas1/2 endonuclease complex reduces CRISPR/Cascade guide array stability Z Ye, J Lebeau, EA Moreb, R Menacho-Melgar, MD Lynch bioRxiv, 2020 | 1 | 2020 |
Simple Scalable Protein Expression and Extraction Using Two-stage Autoinducible Cell Autolysis and DNA/RNA Autohydrolysis in Escherichia coli R Menacho-Melgar, MD Lynch Bio-protocol 12 (2), e4297-e4297, 2022 | | 2022 |
The Development of 2-stage Microfermentation Protocols for High Throughput Cell Factory Evaluations S Li, Z Ye, EA Moreb, R Menacho-Melgar, MD Lynch bioRxiv, 2022 | | 2022 |
TheE. coliCas1/2 endonuclease complex reduces CRISPR/Cascade guide array stability Z Ye, J Lebeau, EA Moreb, R Menacho-Melgar, MD Lynch | | 2020 |
Robustness testing and scalability of phosphate regulated promoters useful for two-stage autoinduction in E. coli EA Moreb, Z Ye, JP Efromson, JN Hennigan, R Menacho-Melgar, ... bioRxiv, 2020 | | 2020 |
Improved, scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion R Menacho-Melgar, Z Ye, EA Moreb, T Yang, JP Efromson, JS Decker, ... bioRxiv, 820787, 2020 | | 2020 |