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Romel Menacho-Melgar
Romel Menacho-Melgar
Duke University
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Year
A review of lipidation in the development of advanced protein and peptide therapeutics
R Menacho-Melgar, JS Decker, JN Hennigan, MD Lynch
Journal of Controlled Release 295, 1-12, 2019
892019
Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity
EA Moreb, B Hoover, A Yaseen, N Valyasevi, Z Roecker, ...
ACS synthetic biology 6 (12), 2209-2218, 2017
482017
Scalable, two‐stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion
R Menacho‐Melgar, Z Ye, EA Moreb, T Yang, JP Efromson, JS Decker, ...
Biotechnology and Bioengineering, 2020
382020
Low-Cost, Large-Scale Production of the Anti-viral Lectin Griffithsin
JS Decker, R Menacho-Melgar, MD Lynch
Frontiers in bioengineering and biotechnology 8, 1020, 2020
302020
Improved, two‐stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease
R Menacho‐Melgar, EA Moreb, JP Efromson, T Yang, JN Hennigan, ...
Biotechnology and Bioengineering, 2020
292020
Media Robustness and Scalability of Phosphate Regulated Promoters Useful for Two-Stage Autoinduction in E. coli
EA Moreb, Z Ye, JP Efromson, JN Hennigan, R Menacho-Melgar, ...
ACS Synthetic Biology, 2020
282020
Escherichia coli Cas1/2 Endonuclease Complex Modifies Self-Targeting CRISPR/Cascade Spacers Reducing Silencing Guide Stability
Z Ye, EA Moreb, S Li, J Lebeau, R Menacho-Melgar, M Munson, ...
ACS Synthetic Biology 10 (1), 29-37, 2020
82020
Optimization of phosphate-limited autoinduction broth for two-stage heterologous protein expression in Escherichia coli
R Menacho-Melgar, JN Hennigan, MD Lynch
Biotechniques 71 (4), 566-572, 2021
72021
Instant Taq: Rapid Autoinducible Expression and Chromatography-free Purification of Taq polymerase
R Menacho-Melgar, T Yang, MD Lynch
bioRxiv, 2021
32021
Media robustness and scalability of phosphate regulated promoters useful for two-stage autoinduction in E. coli. ACS Synthetic Biology, 9, 1483–1486
EA Moreb, Z Ye, JP Efromson, JN Hennigan, R Menacho-Melgar, ...
32020
Integrated autolysis, DNA hydrolysis and precipitation enables an improved bioprocess for Q-Griffithsin, a broad-spectrum antiviral and clinical-stage anti-COVID-19 candidate
JS Decker, R Menacho-Melgar, MD Lynch
Biochemical Engineering Journal 181, 108403, 2022
22022
Measuring Oligonucleotide Hydrolysis in Cellular Lysates via Viscosity Measurements
R Menacho-Melgar, MD Lynch
Bio-protocol 12 (2), e4304-e4304, 2022
12022
Optimization of Phosphate Limited Autoinduction Broth for 2-Stage Heterologous Protein Expression in E. coli
R Menacho-Melgar, JN Hennigan, MD Lynch
bioRxiv, 2021
12021
The E. coli Cas1/2 endonuclease complex reduces CRISPR/Cascade guide array stability
Z Ye, J Lebeau, EA Moreb, R Menacho-Melgar, MD Lynch
bioRxiv, 2020
12020
Simple Scalable Protein Expression and Extraction Using Two-stage Autoinducible Cell Autolysis and DNA/RNA Autohydrolysis in Escherichia coli
R Menacho-Melgar, MD Lynch
Bio-protocol 12 (2), e4297-e4297, 2022
2022
The Development of 2-stage Microfermentation Protocols for High Throughput Cell Factory Evaluations
S Li, Z Ye, EA Moreb, R Menacho-Melgar, MD Lynch
bioRxiv, 2022
2022
TheE. coliCas1/2 endonuclease complex reduces CRISPR/Cascade guide array stability
Z Ye, J Lebeau, EA Moreb, R Menacho-Melgar, MD Lynch
2020
Robustness testing and scalability of phosphate regulated promoters useful for two-stage autoinduction in E. coli
EA Moreb, Z Ye, JP Efromson, JN Hennigan, R Menacho-Melgar, ...
bioRxiv, 2020
2020
Improved, scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion
R Menacho-Melgar, Z Ye, EA Moreb, T Yang, JP Efromson, JS Decker, ...
bioRxiv, 820787, 2020
2020
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Articles 1–19