| Protic ionic liquid as additive on lipase immobilization using silica sol–gel RL de Souza, ELP de Faria, RT Figueiredo, L dos Santos Freitas, ... Enzyme and Microbial Technology 52 (3), 141-150, 2013 | 98 | 2013 |
| Lipase purification using ionic liquids as adjuvants in aqueous two-phase systems RL Souza, SPM Ventura, CMF Soares, JAP Coutinho, ÁS Lima Green Chemistry 17 (5), 3026-3034, 2015 | 96 | 2015 |
| Purification of lipase produced by a new source of Bacillus in submerged fermentation using an aqueous two-phase system JMP Barbosa, RL Souza, AT Fricks, GM Zanin, CMF Soares, ÁS Lima Journal of Chromatography B 879 (32), 3853-3858, 2011 | 89 | 2011 |
| Effect of ionic liquids as adjuvants on PEG-based ABS formation and the extraction of two probe dyes RL de Souza, VC Campos, SPM Ventura, CMF Soares, JAP Coutinho, ... Fluid Phase Equilibria 375, 30-36, 2014 | 88 | 2014 |
| Aqueous two-phase systems based on cholinium salts and tetrahydrofuran and their use for lipase purification RL Souza, RA Lima, JAP Coutinho, CMF Soares, ÁS Lima Separation and Purification Technology 155, 118-126, 2015 | 73 | 2015 |
| Partitioning of porcine pancreatic lipase in a two-phase systems of polyethylene glycol/potassium phosphate aqueous RL de Souza, JMP Barbosa, GM Zanin, MWN Lobão, CMF Soares, ... Applied biochemistry and biotechnology 161, 288-300, 2010 | 62 | 2010 |
| Novel aqueous two-phase systems based on tetrahydrofuran and potassium phosphate buffer for purification of lipase RL Souza, RA Lima, JAP Coutinho, CMF Soares, ÁS Lima Process Biochemistry 50 (9), 1459-1467, 2015 | 45 | 2015 |
| Optimization of the enzymatic hydrolysis of Moringa oleifera Lam oil using molecular docking analysis for fatty acid specificity MS Barbosa, CCC Freire, LC Almeida, LS Freitas, RL Souza, EB Pereira, ... Biotechnology and Applied Biochemistry 66 (5), 823-832, 2019 | 43 | 2019 |
| Recovery of capsaicin from Capsicum frutescens by applying aqueous two-phase systems based on acetonitrile and cholinium-based ionic liquids PL Santos, LNS Santos, SPM Ventura, RL de Souza, JAP Coutinho, ... Chemical Engineering Research and Design 112, 103-112, 2016 | 42 | 2016 |
| Ionic liquid‐based aqueous biphasic systems as a versatile tool for the recovery of antioxidant compounds JH Santos, FA e Silva, SPM Ventura, JAP Coutinho, RL de Souza, ... Biotechnology Progress 31 (1), 70-77, 2015 | 40 | 2015 |
| Influence of the use of Aliquat 336 in the immobilization procedure in sol–gel of lipase from Bacillus sp. ITP-001 RL Souza, WC Resende, CE Barão, GM Zanin, HF de Castro, ... Journal of Molecular Catalysis B: Enzymatic 84, 152-159, 2012 | 39 | 2012 |
| Sequential degradation of raw vinasse by a laccase enzyme producing fungus Pleurotus sajor-caju and its ATPS purification JA Junior, YA Vieira, IA Cruz, D da Silva Vilar, MM Aguiar, NH Torres, ... Biotechnology reports 25, e00411, 2020 | 36 | 2020 |
| Sequential production of amylolytic and lipolytic enzymes by bacterium strain isolated from petroleum contaminated soil NB Carvalho, RL de Souza, HF de Castro, GM Zanin, ÁS Lima, ... Applied biochemistry and biotechnology 150, 25-32, 2008 | 36 | 2008 |
| Integrated process for purification of capsaicin using aqueous two-phase systems based on ethanol NEC Cienfuegos, PL Santos, AR García, CMF Soares, AS Lima, ... Food and Bioproducts Processing 106, 1-10, 2017 | 34 | 2017 |
| Lipase activation by molecular bioimprinting: The role of interactions between fatty acids and enzyme active site LMS Brandao, MS Barbosa, RL Souza, MM Pereira, AS Lima, ... Biotechnology Progress 37 (1), e3064, 2021 | 32 | 2021 |
| Production and characterization of the bio-oil obtained by the fast pyrolysis of spent coffee grounds of the soluble coffee industry MC Krause, AC Moitinho, L Fernando R. Ferreira, RL Souza, LC Krause, ... Journal of the Brazilian Chemical Society 30 (8), 1608-1615, 2019 | 30 | 2019 |
| Use of protic ionic liquids as adjuvants in PEG-based ATPS for the purification of radish peroxidase IV Lucena, IV Brandão, S Mattedi, RL Souza, CMF Soares, AT Fricks, ... Fluid Phase Equilibria 452, 1-8, 2017 | 28 | 2017 |
| Acetonitrile as adjuvant to tune polyethylene glycol+ K3PO4 aqueous two-phase systems and its effect on phenolic compounds partition RL Souza, FO Farias, MR Mafra, CMF Soares, H Passos, JAP Coutinho, ... Separation and Purification Technology 223, 41-48, 2019 | 26 | 2019 |
| Biochemical characterisation of lipase from a new strain of Bacillus sp. ITP-001 JMP Barbosa, RL Souza, CM Melo, AT Fricks, CMF Soares, ÁS Lima Química Nova 35, 1173-1178, 2012 | 26 | 2012 |
| Complete process for the selective recovery of textile dyes using aqueous two-phase system L Alencar, LMS Passos, MAR Martins, IMA Barreto, CMF Soares, AS Lima, ... Separation and Purification Technology 253, 117502, 2020 | 23 | 2020 |
Joao A. P. CoutinhoFull Professor, CICECO, Chemistry Department, University of AveiroVerified email at ua.pt
