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Digvijay Singh
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The SARS-CoV-2 Nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein
S Lu, Q Ye, D Singh, E Villa, DW Cleveland, KD Corbett
Biorxiv, 2020
3602020
Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
D Singh, SH Sternberg, J Fei, JA Doudna, T Ha
Nature Communications 7, 2016
2522016
HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells
H Yu, S Lu, K Gasior, D Singh, S Vazquez-Sanchez, O Tapia, D Toprani, ...
Science 371 (6529), eabb4309, 2021
1972021
Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)
D Singh, J Mallon, A Poddar, Y Wang, R Tippana, O Yang, S Bailey, T Ha
Proceedings of the National Academy of Sciences 115 (21), 5444-5449, 2018
1632018
An improved surface passivation method for single-molecule studies
B Hua, KY Han, R Zhou, H Kim, X Shi, SC Abeysirigunawardena, A Jain, ...
Nature methods 11 (12), 1233-1236, 2014
1622014
Protein stability and folding kinetics in the nucleus and endoplasmic reticulum of eucaryotic cells
A Dhar, K Girdhar, D Singh, H Gelman, S Ebbinghaus, M Gruebele
Biophysical journal 101 (2), 421-430, 2011
1572011
Determination of in vivo target search kinetics of regulatory noncoding RNA
J Fei, D Singh, Q Zhang, S Park, D Balasubramanian, I Golding, ...
Science 347 (6228), 1371-1374, 2015
154*2015
Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography
FR Wagner, R Watanabe, R Schampers, D Singh, H Persoon, M Schaffer, ...
Nature protocols 15 (6), 2041-2070, 2020
1192020
Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis
D Singh, Y Wang, J Mallon, O Yang, J Fei, A Poddar, D Ceylan, S Bailey, ...
Nature structural & molecular biology 25 (4), 347, 2018
1082018
Comprehensive structure and functional adaptations of the yeast nuclear pore complex
CW Akey*, D Singh*, C Ouch*, I Echeverria*, I Nudelman, JM Varberg, ...
Cell 185 (2), *Co-first authors, 2022
912022
Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding
TH Ikenna Okafor*, Digvijay Singh*, Yanbo Wang*, Minhee Jung, Haobo Wang ...
Nucleic Acids Research, *co-first author, 2019
382019
Real-time observation of Cas9 postcatalytic domain motions
Y Wang, J Mallon, H Wang, D Singh, M Hyun Jo, B Hua, S Bailey, T Ha
Proceedings of the National Academy of Sciences 118 (2), e2010650118, 2021
202021
The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein. Nat Commun 12: 502
S Lu, Q Ye, D Singh, Y Cao, JK Diedrich, JR Yates, E Villa, DW Cleveland, ...
152021
The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein. Nat Commun. 2021; 12 (1): 502
S Lu, Q Ye, D Singh, Y Cao, JK Diedrich, JR Yates, E Villa, DW Cleveland, ...
Epub 2021/01/23. https://doi. org/10.1038/s41467-020-20768-y PMID: 33479198, 2021
152021
HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells. Science 371, eabb4309
H Yu, S Lu, K Gasior, D Singh, S Vazquez-Sanchez, O Tapia, D Toprani, ...
142021
Understanding the molecular mechanisms of CRISPR toolbox using single molecule approaches
D Singh, T Ha
ACS Chemical Biology, 2018
132018
A viral genome packaging ring-ATPase is a flexibly coordinated pentamer
L Dai*, D Singh*, S Lu, VI Kottadiel, R Vafabakhsh, M Mahalingam, ...
Nature communications 12 (1), *Co-first author, 2021
112021
The single-molecule centroid localization algorithm improves the accuracy of fluorescence binding assays
B Hua, Y Wang, S Park, KY Han, D Singh, JH Kim, W Cheng, T Ha
Biochemistry 57 (10), 1572-1576, 2018
92018
Effects of individual base-pairs on in vivo target search and destruction kinetics of small RNA
A Poddar, MS Azam, T Kayikcioglu, M Bobrovskyy, J Zhang, X Ma, ...
7*2020
TDP-43 and HSP70 phase separate into anisotropic, intranuclear liquid spherical annuli
H Yu, S Lu, K Gasior, D Singh, O Tapia, S Vazquez-Sanchez, D Toprani, ...
bioRxiv, 2020.03. 28.985986, 2020
52020
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