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Nathan E. Thomas
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New free-exchange model of EmrE transport
AE Robinson, NE Thomas, EA Morrison, BM Balthazor, ...
Proceedings of the National Academy of Sciences 114 (47), E10083-E10091, 2017
562017
Structure and dynamics of the drug-bound bacterial transporter EmrE in lipid bilayers
AA Shcherbakov, G Hisao, VS Mandala, NE Thomas, M Soltani, EA Salter, ...
Nature Communications 12 (1), 172, 2021
452021
Highly coupled transport can be achieved in free-exchange transport models
GA Hussey, NE Thomas, KA Henzler-Wildman
Journal of General Physiology 152 (1), e201912437, 2019
272019
Identification of an alternating-access dynamics mutant of EmrE with impaired transport
C Wu, SA Wynne, NE Thomas, EM Uhlemann, CG Tate, ...
Journal of molecular biology 431 (15), 2777-2789, 2019
202019
The C terminus of the bacterial multidrug transporter EmrE couples drug binding to proton release
NE Thomas, C Wu, EA Morrison, AE Robinson, JP Werner, ...
Journal of Biological Chemistry 293 (49), 19137-19147, 2018
182018
Estimates of RelSeq, Mesh1, and SAHMex hydrolysis of (p) ppGpp and (p) ppApp by thin layer chromatography and NADP/NADH coupled assays
K Potrykus, NE Thomas, B Bruhn-Olszewska, M Sobala, M Dylewski, ...
Frontiers in Microbiology 11, 581271, 2020
152020
A solid-supported membrane electrophysiology assay for efficient characterization of ion-coupled transport
NE Thomas, W Feng, KA Henzler-Wildman
Journal of Biological Chemistry 297 (4), 2021
122021
Activating alternative transport modes in a multidrug resistance efflux pump to confer chemical susceptibility
PJ Spreacker, NE Thomas, WF Beeninga, M Brousseau, CJ Porter, ...
Nature Communications 13 (1), 7655, 2022
62022
Proceedings of the National Academy of Sciences of the United States of America
CK Johnson, LG Tassinary
22007
A new substrate triggers susceptibility by uncoupling a bacterial multidrug resistance efflux pump
PJ Spreacker, NE Thomas, WF Beeninga, M Brousseau, KM Hibbs, ...
bioRxiv, 2021.12. 04.471113, 2021
12021
Unlocking the reversal potential of solid supported membrane electrophysiology to determine transport stoichiometry
NE Thomas, KA Henzler-Wildman
bioRxiv, 2020.05. 07.082438, 2020
12020
A kinetically-driven free exchange mechanism of EmrE antiport sacrifices coupling efficiency in favor of promiscuity
AE Robinson, NE Thomas, EA Morrison, B Balthazor, ...
bioRxiv, 141937, 2017
12017
Using solid-supported membrane electrophysiology to probe the transport mechanism of EmrE
M Brousseau, NE Thomas, PJ Spreacker, KA Henzler-Wildman
Biophysical Journal 122 (3), 400a, 2023
2023
Can Complete Transporter Mechanisms be Inferred from Experimental Data?
A George, NE Thomas, KA Henzler-Wildman, DM Zuckerman
Biophysical Journal 120 (3), 85a, 2021
2021
The Proton/Drug Coupling Mechanism of EmrE
NE Thomas
The University of Wisconsin-Madison, 2021
2021
Rethinking the Bounds of Ion-Coupled Transport
NE Thomas, G Hussey, KA Henzler-Wildman
Biophysical Journal 118 (3), 130a, 2020
2020
Controlling the Rate and Efficiency of Proton-Coupled Transport by EmrE
N Thomas, C Wu, P Spreacker, G Hussey, S Wynne, EM Uhlemann, ...
Biophysical Journal 118 (3), 2a, 2020
2020
A free‐exchange mathematical model of EmrE
G Hussey, N Thomas, K Henzler‐Wildman
The FASEB Journal 33 (S1), 656.6-656.6, 2019
2019
Sacrificing efficiency to achieve promiscuity: re-examining the multi drug transport mechanism of EmrE
K Henzler-Wildman, A Robinson, N Thomas, E Morrison
FEBS JOURNAL 284, 15-15, 2017
2017
Determination of EmrE’s Proton/Drug Coupling Stoichiometry
N Thomas, K Henzler-Wildman
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Articles 1–20