| Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids AV Bryksin, I Matsumura Biotechniques 48 (6), 463-465, 2010 | 724 | 2010 |
| Aptamers as therapeutic and diagnostic reagents: problems and prospects SE Osborne, I Matsumura, AD Ellington Current opinion in chemical biology 1 (1), 5-9, 1997 | 388 | 1997 |
| In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates I Matsumura, AD Ellington Journal of molecular biology 305 (2), 331-339, 2001 | 259 | 2001 |
| Multicopy suppression underpins metabolic evolvability WM Patrick, EM Quandt, DB Swartzlander, I Matsumura Molecular biology and evolution 24 (12), 2716-2722, 2007 | 203 | 2007 |
| Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria AV Bryksin, I Matsumura PloS one 5 (10), e13244, 2010 | 158 | 2010 |
| Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli MR Parikh, DN Greene, KK Woods, I Matsumura Protein Engineering Design and Selection 19 (3), 113-119, 2006 | 146 | 2006 |
| Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of β-fucosidase from β-galactosidase MR Parikh, I Matsumura Journal of molecular biology 352 (3), 621-628, 2005 | 127 | 2005 |
| Rapid evolution of β-glucuronidase specificity by saturation mutagenesis of an active site loop ML Geddie, I Matsumura Journal of Biological Chemistry 279 (25), 26462-26468, 2004 | 107 | 2004 |
| Directed evolution of the surface chemistry of the reporter enzyme β-glucuronidase I Matsumura, JB Wallingford, NK Surana, PD Vize, AD Ellington Nature biotechnology 17 (7), 696-701, 1999 | 97 | 1999 |
| Artificially evolved Synechococcus PCC6301 Rubisco variants exhibit improvements in folding and catalytic efficiency DN Greene, SM Whitney, I Matsumura Biochemical Journal 404 (3), 517-524, 2007 | 95 | 2007 |
| Natural history as a predictor of protein evolvability TL O'Loughlin, WM Patrick, I Matsumura Protein Engineering, Design and Selection 19 (10), 439-442, 2006 | 82 | 2006 |
| Is aspartate 52 essential for catalysis by chicken egg white lysozyme? The role of natural substrate-assisted hydrolysis I Matsumura, JF Kirsch Biochemistry 35 (6), 1881-1889, 1996 | 78 | 1996 |
| A comparison of directed evolution approaches using the β-glucuronidase model system LA Rowe, ML Geddie, OB Alexander, I Matsumura Journal of molecular biology 332 (4), 851-860, 2003 | 72 | 2003 |
| A study in molecular contingency: glutamine phosphoribosylpyrophosphate amidotransferase is a promiscuous and evolvable phosphoribosylanthranilate isomerase WM Patrick, I Matsumura Journal of molecular biology 377 (2), 323-336, 2008 | 64 | 2008 |
| Fluorine-19 nuclear magnetic resonance spectroscopic study of fluorophenylalanine-and fluorotryptophan-labeled avian egg white lysozymes C Lian, H Le, B Montez, J Patterson, S Harrell, D Laws, I Matsumura, ... Biochemistry 33 (17), 5238-5245, 1994 | 61 | 1994 |
| Diversification and specialization of HIV protease function during in vitro evolution TL O'Loughlin, DN Greene, I Matsumura Molecular biology and evolution 23 (4), 764-772, 2006 | 50 | 2006 |
| Overlap extension PCR cloning A Bryksin, I Matsumura Synthetic biology, 31-42, 2013 | 49 | 2013 |
| Expression vectors for Acinetobacter baylyi ADP1 CD Murin, K Segal, A Bryksin, I Matsumura Applied and environmental microbiology 78 (1), 280-283, 2012 | 44 | 2012 |
| Assays for identifying functional alterations in the p53 tumor suppressor AD Ellington, I Matsumura US Patent 6,429,298, 2002 | 41 | 2002 |
| In vitro evolution of thermostable p53 variants I Matsumura, AD Ellington Protein science 8 (4), 731-740, 1999 | 41 | 1999 |
