| Generation of high-affinity DNA aptamers using an expanded genetic alphabet M Kimoto, R Yamashige, K Matsunaga, S Yokoyama, I Hirao Nature biotechnology 31 (5), 453-457, 2013 | 531 | 2013 |
| An unnatural base pair for incorporating amino acid analogs into proteins I Hirao, T Ohtsuki, T Fujiwara, T Mitsui, T Yokogawa, T Okuni, ... Nature biotechnology 20 (2), 177-182, 2002 | 379 | 2002 |
| Site‐specific incorporation of an unnatural amino acid into proteins in mammalian cells K Sakamoto, A Hayashi, A Sakamoto, D Kiga, H Nakayama, A Soma, ... Nucleic acids research 30 (21), 4692-4699, 2002 | 358 | 2002 |
| An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA I Hirao, M Kimoto, T Mitsui, T Fujiwara, R Kawai, A Sato, Y Harada, ... Nature Methods 3 (9), 729-735, 2006 | 295 | 2006 |
| Most compact hairpin-turn structure exerted by a short DNA fragment, d (GCGAAGC) in solution: an extraordinarily stable structure resistant to nucleases and heat I Hirao, G Kawai, S Yoshizawa, Y Nishimura, Y Ishido, K Watanabe, ... Nucleic acids research 22 (4), 576-582, 1994 | 247 | 1994 |
| Unnatural base pair systems for DNA/RNA-based biotechnology I Hirao Current opinion in chemical biology 10 (6), 622-627, 2006 | 218 | 2006 |
| An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules M Kimoto, R Kawai, T Mitsui, S Yokoyama, I Hirao Nucleic acids research 37 (2), e14-e14, 2009 | 216 | 2009 |
| An engineered Escherichia coli tyrosyl–tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its … D Kiga, K Sakamoto, K Kodama, T Kigawa, T Matsuda, T Yabuki, ... Proceedings of the National Academy of Sciences 99 (15), 9715-9720, 2002 | 209 | 2002 |
| Highly specific unnatural base pair systems as a third base pair for PCR amplification R Yamashige, M Kimoto, Y Takezawa, A Sato, T Mitsui, S Yokoyama, ... Nucleic acids research 40 (6), 2793-2806, 2012 | 183 | 2012 |
| Natural versus artificial creation of base pairs in DNA: Origin of nucleobases from the perspectives of unnatural base pair studies I Hirao, M Kimoto, R Yamashige Accounts of chemical research 45 (12), 2055-2065, 2012 | 179 | 2012 |
| GNA trinucleotide loop sequences producing extraordinarily stable DNA minihairpins S Yoshizawa, G Kawai, K Watanabe, K Miura, I Hirao Biochemistry 36 (16), 4761-4767, 1997 | 176 | 1997 |
| Extraordinarily stable mini-hairpins: electrophoretical and thermal properties of the various sequence variants of d (GCFAAAGC) and their effect on DNA sequencing I Hirao, Y Nishimura, Y Tagawa, K Watanabe, K Miura Nucleic acids research 20 (15), 3891-3896, 1992 | 167 | 1992 |
| A two-unnatural-base-pair system toward the expansion of the genetic code I Hirao, Y Harada, M Kimoto, T Mitsui, T Fujiwara, S Yokoyama Journal of the American Chemical Society 126 (41), 13298-13305, 2004 | 139 | 2004 |
| An unnatural hydrophobic base pair with shape complementarity between pyrrole-2-carbaldehyde and 9-methylimidazo [(4, 5)-b] pyridine T Mitsui, A Kitamura, M Kimoto, T To, A Sato, I Hirao, S Yokoyama Journal of the American Chemical Society 125 (18), 5298-5307, 2003 | 138 | 2003 |
| High-affinity DNA aptamer generation targeting von Willebrand factor A1-domain by genetic alphabet expansion for systematic evolution of ligands by exponential enrichment using … K Matsunaga, M Kimoto, I Hirao Journal of the American Chemical Society 139 (1), 324-334, 2017 | 133 | 2017 |
| An efficient unnatural base pair for PCR amplification I Hirao, T Mitsui, M Kimoto, S Yokoyama Journal of the American Chemical Society 129 (50), 15549-15555, 2007 | 130 | 2007 |
| Unnatural base pairs for specific transcription T Ohtsuki, M Kimoto, M Ishikawa, T Mitsui, I Hirao, S Yokoyama Proceedings of the National Academy of Sciences 98 (9), 4922-4925, 2001 | 129 | 2001 |
| Site-specific fluorescent labeling of RNA molecules by specific transcription using unnatural base pairs R Kawai, M Kimoto, S Ikeda, T Mitsui, M Endo, S Yokoyama, I Hirao Journal of the American Chemical Society 127 (49), 17286-17295, 2005 | 127 | 2005 |
| Crystal structures of a series of RNA aptamers complexed to the same protein target S Rowsell, NJ Stonehouse, MA Convery, CJ Adams, AD Ellington, I Hirao, ... Nature structural biology 5 (11), 970-975, 1998 | 126 | 1998 |
| Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma I Hirao, M Kimoto Proceedings of the Japan Academy, Series B 88 (7), 345-367, 2012 | 92 | 2012 |
