Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state ZJ Liu, GA Stepanyuk, ES Vysotski, J Lee, SV Markova, NP Malikova, ... Proceedings of the National Academy of Sciences 103 (8), 2570-2575, 2006 | 104 | 2006 |
Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein GA Stepanyuk, S Golz, SV Markova, LA Frank, J Lee, ES Vysotski FEBS letters 579 (5), 1008-1014, 2005 | 92 | 2005 |
Transient protein-RNA interactions guide nascent ribosomal RNA folding O Duss, GA Stepanyuk, JD Puglisi, JR Williamson Cell 179 (6), 1357-1369. e16, 2019 | 77 | 2019 |
Coelenterazine-binding protein of Renilla muelleri: cDNA cloning, overexpression, and characterization as a substrate of luciferase MS Titushin, SV Markova, LA Frank, NP Malikova, GA Stepanyuk, J Lee, ... Photochemical & Photobiological Sciences 7 (2), 189-196, 2008 | 68 | 2008 |
NMR-derived topology of a GFP-photoprotein energy transfer complex MS Titushin, GA Stepanyuk, SV Markova, S Golz, J Lee, ES Vysotski Journal of biological chemistry 285 (52), 40891-40900, 2010 | 56 | 2010 |
Violet and greenish photoprotein obelin mutants for reporter applications in dual-color assay LA Frank, VV Borisova, SV Markova, NP Malikova, GA Stepanyuk, ... Analytical and bioanalytical chemistry 391, 2891-2896, 2008 | 54 | 2008 |
Coelenterazine-v ligated to Ca2+-triggered coelenterazine-binding protein is a stable and efficient substrate of the red-shifted mutant of Renilla muelleri luciferase GA Stepanyuk, J Unch, NP Malikova, SV Markova, J Lee, ES Vysotski Analytical and bioanalytical chemistry 398, 1809-1817, 2010 | 53 | 2010 |
Spectral tuning of obelin bioluminescence by mutations of Trp92 NP Malikova, GA Stepanyuk, LA Frank, SV Markova, ES Vysotski, J Lee FEBS letters 554 (1-2), 184-188, 2003 | 52 | 2003 |
Expression, purification and characterization of the secreted luciferase of the copepod Metridia longa from Sf9 insect cells GA Stepanyuk, H Xu, CK Wu, SV Markova, J Lee, ES Vysotski, BC Wang Protein expression and purification 61 (2), 142-148, 2008 | 50 | 2008 |
Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 Å: Why it is not a calcium-regulated photoprotein GA Stepanyuk, ZJ Liu, SS Markova, LA Frank, J Lee, ES Vysotski, ... Photochemical & Photobiological Sciences 7 (4), 442-447, 2008 | 46 | 2008 |
Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts O Duss, GA Stepanyuk, A Grot, SE O’Leary, JD Puglisi, JR Williamson Nature communications 9 (1), 5087, 2018 | 44 | 2018 |
UHM–ULM interactions in the RBM39–U2AF65 splicing-factor complex GA Stepanyuk, P Serrano, E Peralta, CL Farr, HL Axelrod, M Geralt, ... Acta Crystallographica Section D: Structural Biology 72 (4), 497-511, 2016 | 41 | 2016 |
Spatial structure of the novel light-sensitive photoprotein berovin from the ctenophore Beroe abyssicola in the Ca2+-loaded apoprotein conformation state GA Stepanyuk, ZJ Liu, LP Burakova, J Lee, J Rose, ES Vysotski, ... Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1834 (10), 2139-2146, 2013 | 34 | 2013 |
Bioluminescent detection probe for tick-borne encephalitis virus immunoassay LP Burakova, AN Kudryavtsev, GA Stepanyuk, IK Baykov, VV Morozova, ... Analytical and bioanalytical chemistry 407, 5417-5423, 2015 | 26 | 2015 |
Structure based mechanism of the Ca2+‐induced release of coelenterazine from the Renilla binding protein GA Stepanyuk, ZJ Liu, ES Vysotski, J Lee, JP Rose, BC Wang Proteins: Structure, Function, and Bioinformatics 74 (3), 583-593, 2009 | 24 | 2009 |
Role of certain amino acid residues of the coelenterazine-binding cavity in bioluminescence of light-sensitive Ca2+-regulated photoprotein berovin LP Burakova, GA Stepanyuk, EV Eremeeva, ES Vysotski Photochemical & Photobiological Sciences 15 (5), 691-704, 2016 | 17 | 2016 |
Ca2+-triggered coelenterazine-binding protein from Renilla as an enzyme-dependent label for binding assay VV Krasitskaya, SI Korneeva, AN Kudryavtsev, SV Markova, ... Analytical and bioanalytical chemistry 401, 2573-2579, 2011 | 10 | 2011 |
Engineering of obelin with changed calcium affinities and light emission spectra for simultaneous measurement of [Ca2+] in cytoplasm and mitochondria NP Malikova, LP Burakova, AV Eremeev, SV Markova, GA Stepanyuk, ... Journal of Biotechnology, 429, 2010 | 3 | 2010 |
APOOBELIN BIOTINYLATED IN VIVO: OVERPRODUCTION IN ESCHERICHIA COLI CELLS SV Markova, GA Stepanyuk, LA Frank, ES Vysotski Bioluminescence And Chemiluminescence: Progress and Current Applications …, 2002 | 3 | 2002 |
Isolated Photoprotein Aqdecay, and Its Use S Golz, E Vysotski, S Markova, GA Stepanyuk, L Frank US Patent App. 11/920,386, 2009 | 2 | 2009 |