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Andreas Pluckthun
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In vitro selection and evolution of functional proteins by using ribosome display
J Hanes, A Plückthun
Proceedings of the National Academy of Sciences 94 (10), 4937-4942, 1997
18131997
Assembly of a Functional Immunoglobulin Fv Fragment in Escherichia coli
A Skerra, A Plückthun
Science 240 (4855), 1038-1041, 1988
16781988
Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides
A Knappik, L Ge, A Honegger, P Pack, M Fischer, GÈ Wellnhofer, A Hoess, ...
Journal of molecular biology 296 (1), 57-86, 2000
11892000
Engineering novel binding proteins from nonimmunoglobulin domains
HK Binz, P Amstutz, A Plückthun
Nature biotechnology 23 (10), 1257-1268, 2005
9572005
Induction and exhaustion of lymphocytic choriomeningitis virus–specific cytotoxic T lymphocytes visualized using soluble tetrameric major histocompatibility complex class I …
A Gallimore, A Glithero, A Godkin, AC Tissot, A Plückthun, T Elliott, ...
The Journal of experimental medicine 187 (9), 1383-1393, 1998
9231998
High-affinity binders selected from designed ankyrin repeat protein libraries
HK Binz, P Amstutz, A Kohl, MT Stumpp, C Briand, P Forrer, MG Grütter, ...
Nature biotechnology 22 (5), 575-582, 2004
8472004
In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei
C Schaffitzel, I Berger, J Postberg, J Hanes, HJ Lipps, A Plückthun
Proceedings of the National Academy of Sciences 98 (15), 8572-8577, 2001
8172001
Stability engineering of antibody single-chain Fv fragments
A Wörn, A Plückthun
Journal of molecular biology 305 (5), 989-1010, 2001
8102001
A comparison of strategies to stabilize immunoglobulin Fv-fragments
R Glockshuber, M Malia, I Pfitzinger, A Plueckthun
Biochemistry 29 (6), 1362-1367, 1990
7411990
Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins
HK Binz, MT Stumpp, P Forrer, P Amstutz, A Plückthun
Journal of molecular biology 332 (2), 489-503, 2003
7382003
Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system
A Krebber, S Bornhauser, J Burmester, A Honegger, J Willuda, ...
Journal of immunological methods 201 (1), 35-55, 1997
6901997
Reproducibility: Standardize antibodies used in research
A Bradbury, A Plückthun
Nature 518 (7537), 27-29, 2015
6762015
Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy
A Plückthun
Annual review of pharmacology and toxicology 55, 489-511, 2015
5992015
Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display
J Hanes, C Schaffitzel, A Knappik, A Plückthun
Nature biotechnology 18 (12), 1287-1292, 2000
5912000
New protein engineering approaches to multivalent and bispecific antibody fragments
A Plückthun, P Pack
Immunotechnology 3 (2), 83-105, 1997
5501997
In-vitro protein evolution by ribosome display and mRNA display
D Lipovsek, A Plückthun
Journal of immunological methods 290 (1-2), 51-67, 2004
5282004
Miniantibodies: use of amphipathic helixes to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli
P Pack, A Plückthun
Biochemistry 31 (6), 1579-1584, 1992
5001992
Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries
J Hanes, L Jermutus, S Weber-Bornhauser, HR Bosshard, A Plückthun
Proceedings of the National Academy of Sciences 95 (24), 14130-14135, 1998
4821998
Biophysical properties of human antibody variable domains
S Ewert, T Huber, A Honegger, A Plückthun
Journal of molecular biology 325 (3), 531-553, 2003
4662003
BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip
L Nieba, SE Nieba-Axmann, A Persson, M Hämäläinen, F Edebratt, ...
Analytical biochemistry 252 (2), 217-228, 1997
4611997
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