Multicellular organisms have the challenging task of coordinating the activities of many
distinct cell types. This coordination is accomplished largely by cell-associated and soluble
signalling molecules that act locally or distantly to alter target-cell physiology. The tumour …

In addition to the Trk tyrosine kinase receptors, neurotrophins also bind to a second
receptor, p75, a member of the tumor necrosis factor receptor superfamily. Several signaling
pathways have been implicated for p75 in the absence of Trk receptors, including induction …

CD95 (Fas/Apo-1), a member of the nerve growth factor/tumor necrosis factor (NGF/TNF)
receptor superfamily, initiates a signal-transduction cascade leading to programmed cell
death. The extracellular portion of the CD95 receptor contains three cysteine-rich domains …

The Fas receptor rapidly induces apoptosis when activated by ligand binding or by cross-
linking with anti-Fas antibody. The Fas ligand (FasL), a member of the tumor necrosis factor
family of ligands, is a 40-kilodalton type II transmembrane protein which is cleaved to …

Fas is a member of the TNF receptor family, that contain 2–6 cysteine-rich domains (CRDs)
in their extracellular regions, a single transmembrane domain and variably sized
intracytoplasmic domains. Fas belongs to a subgroup of family members that have a “death …

The Fas receptor is a member of a family of cell death receptors, including tumor necrosis
factor receptor I (TNFR I), death receptor 3 and 4 (DR3 and DR4), and cytopathic avian
receptor 1 (CAR1). The Fas receptor is composed of several discrete domains, including …

The p75 neurotrophic receptor (p75) shares structural features with the Fas receptor (FasR).
Both receptors contain extracellular cysteine-rich repeats, a single transmembrane domain,
and intracellular death domains. However, it has not been clearly established whether their …

Glutathione-S-transferase (GST) fusion proteins have had a wide range of applications since
their introduction as tools for synthesis of recombinant proteins in bacteria. GST was
originally selected as a fusion moiety because of several desirable properties. First and …

METHOD 1. Incubate the cell lysate with 50 μL of glutathione-Sepharose beads (50/50 slurry
in lysis buffer) and 25 μg of GST (NOT the GST fusion probe protein) for 2 h at 4 C with end-
over-end mixing. Allow enough volume in the tube to permit liberal mixing; 500 μl to 1 mL is …