Reverse genetics aims to identify the function of a gene with known sequence by phenotypic
analysis of cells or organisms in which the function of this gene is impaired. Commonly used
strategies for reverse genetics encompass transposon mutagenesis (Tissier et al., 1999) and …

In all plant species studied to date, sucrose synthase occurs as multiple isoforms. The
specific functions of the different isoforms are for the most part not clear. Six isoforms of
sucrose synthase have been identified in the model legume Lotus japonicus, the same …

Neutral/alkaline invertases are a subgroup, confined to plants and cyanobacteria, of a
diverse family of enzymes. A family of seven closely-related genes, LjINV1–LjINV7, is
described here and their expression in the model legume, Lotus japonicus, is examined …

We have established tools for forward and reverse genetic analysis of the legume Lotus
(Lotus japonicus). A structured population of M2 progeny of 4,904 ethyl methanesulfonate-
mutagenized M1 embryos is available for single nucleotide polymorphism mutation …

Abstract Characterization of Pisum (pea) seed trypsin inhibitors (TI) and their corresponding
cDNAs indicates that the pea TI gene family contains two genes. The existence of multiple TI
isoforms can be attributed to post‐translational modifications of primary gene products. Post …

Seed trypsin inhibitors have been purified from Pisum. Three groups of inhibitor were
separated using column chromatography, whereas non-denaturing activity gels showed that
five inhibitors could be distinguished. N-terminal sequence data obtained for two purified …

In an attempt to generate more variation of the type represented by the rugosus loci, r and rb,
putative seed development mutants have been isolated from peas (Pisum sativum L.)
following chemical mutagenesis of a round‐seeded (RRRbRb) genotype. The populations …

The metabolism of starch is of central importance for many aspects of plant growth and
development. Information on leaf starch metabolism other than in Arabidopsis is scarce.
Furthermore, its importance in several agronomically important traits exemplified by legumes …

A survey of Pisum genotypes for seed trypsin inhibitors revealed a tenfold range in the
extent of inhibition. Approximately 90% of trypsin inhibitory activity was associated with two
albumin fractions in selected variant lines. The differences among extreme variants were …

Two pairs of near‐isogenic lines of peas were developed to have high and low
concentrations of seed trypsin inhibitor. HA5 and LA5 contained 8.73±0.19 and 1.45±0.19
trypsin inhibitor units per mg dry weight respectively, whereas HB5 and LB5 contained …