Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of
intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the
AKT protein kinase (or protein kinase B), tagged with the green fluorescent protein (PHAKT …

Genetically encodable optical reporters, such as green fluorescent protein, have
revolutionized the observation and measurement of cellular states. However, the inverse
challenge of using light to control precisely cellular behaviour has only recently begun to be …

When presented with a gradient of chemoattractant, many eukaryotic cells respond with
polarized accumulation of the phospholipid PtdIns (3, 4, 5) P 3. This lipid asymmetry is one
of the earliest readouts of polarity in neutrophils, Dictyostelium discoideum and fibroblasts …

In gradients of external chemo-attractant, mammalian neutrophilic leukocytes (neutrophils) 1
and Dictyostelium discoideum amoebae 2 adopt a polarized morphology and selectively
accumulate lipid products of phosphatidylinositol-3-OH kinases (PI (3) Ks), including PtdIns …

Morphologic polarity is necessary for the motility of mammalian cells. In leukocytes
responding to a chemoattractant, this polarity is regulated by activities of small Rho
guanosine triphosphatases (Rho GTPases) and the phosphoinositide 3-kinases (PI3Ks) …

Although many of the regulators of actin assembly are known, we do not understand how
these components act together to organize cell shape and movement. To address this
question, we analyzed the spatial dynamics of a key actin regulator—the Scar/WAVE …

Little is known about how neutrophils and other cells establish a single zone of actin
assembly during migration. A widespread assumption is that the leading edge prevents
formation of additional fronts by generating long-range diffusible inhibitors or by …

Phosphatidylinositol 3-kinase lipid products and the Rho GTPases play a central role in
transmitting information from chemotactic receptors to the effectors of cell polarity, and recent
advances in the field have allowed us to understand these roles more clearly. Emergent …

Persistent directional movement of neutrophils in shallow chemotactic gradients raises the
possibility that cells can increase their sensitivity to the chemotactic signal at the front,
relative to the back. Redistribution of chemoattractant receptors to the anterior pole of a …

Neutrophils respond to chemotactic stimuli by increasing the nucleation and polymerization
of actin filaments, but the location and regulation of these processes are not well
understood. Here, using a permeabilized-cell assay, we show that chemotactic stimuli cause …