Amplification and sequencing of DNA from fungal herbarium specimens
We have utilized the polymerase chain reaction to amplify DNA from 35 collections of dried
fungi obtained from four different herbaria. Sixteen of these samples were subsequently
sequenced. The collections had been dried and stored under a variety of conditions for 1–50
years. DNA was extracted from 5–30 mg of ground tissue and a specific fragment of the
mitochondrial large subunit ribosomal RNA gene was amplified. Sequences obtained from
DNA cloned from a culture and those from DNA amplified from the corresponding dried …
fungi obtained from four different herbaria. Sixteen of these samples were subsequently
sequenced. The collections had been dried and stored under a variety of conditions for 1–50
years. DNA was extracted from 5–30 mg of ground tissue and a specific fragment of the
mitochondrial large subunit ribosomal RNA gene was amplified. Sequences obtained from
DNA cloned from a culture and those from DNA amplified from the corresponding dried …
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