Use of cryoprotectant to maintain long-term peptide immunoreactivity and tissue morphology

RE Watson, SJ Wiegand, RW Clough, GE Hoffman - Peptides, 1986 - Elsevier
Abstract Use of an ethylene glycol based cryoprotectant solution has been found to be
effective for the long-term storage of brain tissue either in block form or as freely floating
sections prior to immunocytochemical processing. Storage of tissue in the solution at− 20 ...

The relevance of cryoprotectant “toxicity” to cryobiology

GM Fahy - Cryobiology, 1986 - Elsevier
Abstract Cryoprotective agents are essential for the cryopreservation of almost all biological
systems. These additives, however, do not usually permit 100% survival after freezing and
thawing, though from a theoretical point of view they should be able to fully suppress all ...

Osmotic consequences of cryoprotectant permeability and its relation to the survival of frozen-thawed embryos

U Schneider, P Mazur - Theriogenology, 1984 - Elsevier
Abstract The freezing of a living cell involves a complex physicochemical process of heat
and water transport between the cell and its surrounding medium. Embryos survive
cryopreservation only in the presence of a cryoprotectant in concentrations between 1 and ...

Proline: a novel cryoprotectant for the freeze preservation of cultured cells of Zea mays L

LA Withers, PJ King - Plant Physiology, 1979 - Am Soc Plant Biol
Abstract Proline is an effective cryoprotectant for the storage of cultured cells of Zea mays L.
in liquid N 2. Increased freeze tolerance can be achieved by pregrowth for 3 to 4 days in
medium containing proline. Cells cryoprotected with proline have an increased recovery ...

Vitrification of immature porcine oocytes: effects of lipid droplets, temperature, cytoskeleton, and addition and removal of cryoprotectant

V Isachenko, C Soler, E Isachenko, F Perez-Sanchez… - Cryobiology, 1998 - Elsevier
Three experiments were conducted to investigate the effects of single-step and stepwise
exposure to and removal of cryoprotectant, of temperature, and of a cytoskeletal relaxant on
the development of germinal vesicle porcine oocytes to the M-II stage. In experiment I, ...

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

GM Beattie, JH Crowe, AD Lopez, V Cirulli… - Diabetes, 1997 - Am Diabetes Assoc
Abstract The scarcity of available tissue for transplantation in diabetes and the need for
multiple donors make it mandatory to use an optimal cryopreservation method that allows
maximal recovery and preservation of β-cell function. We have developed a method to ...

Cryoprotectant toxicity and cryoprotectant toxicity reduction: in search of molecular mechanisms

GM Fahy, TH Lilley, H Linsdell, MSJ Douglas… - Cryobiology, 1990 - Elsevier
Abstract Cryoprotectant toxicity is a fundamental obstacle to the full potential of artificial
cryoprotection, yet it remains in general a poorly understood phenomenon. Unfortunately,
most relevant biochemical studies to date have not met the basic criteria required for ...

Comparison of glycerol, other polyols, trehalose, and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation

BT Storey, EE Noiles, KA Thompson - Cryobiology, 1998 - Elsevier
Most procedures for mouse sperm cryopreservation have utilized raffinose to provide
hypertonicity for cell desiccation prior to freezing and glycerol to block intracellular ice
formation. Trehalose has been shown in other cell systems to provide positive protection ...

Cryoprotectant-induced redistribution of intramembranous particles in mouse lymphocytes

JA McIntyre, NB Gilula, MJ Karnovsky - The Journal of cell biology, 1974 - jcb.rupress.org
Abstract This study demonstrates, by freeze fracture, clustering of intramembranous particles
caused by cryoprotectant treatment of intact unfixed mouse lymphoid cells. Both T and B
cells react in a similar fashion, while similar clustering of particles is not observed in some ...

Effect of cryoprotectant solutes on water permeability of human spermatozoa.

JA Gilmore, LE McGann, J Liu, DY Gao… - Biology of …, 1995 - Soc Study Reprod
Abstract Osmotic permeability characteristics and the effects of cryoprotectants are important
determinants of recovery and function of spermatozoa after cryopreservation. The primary
purpose of this study was to determine the osmotic permeability parameters of human ...

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