Single-molecule PCR using water-in-oil emulsion

M Nakano, J Komatsu, S Matsuura, K Takashima… - Journal of …, 2003 - Elsevier
Polymerase chain reaction (PCR) using a single molecule of DNA is very useful for analysis,
detection and cloning of the desired DNA fragment. We developed a simple PCR method
utilizing a water-in-oil (W/O) emulsion that included numerous droplets of reaction mixture in …