[PDF][PDF] Directed in vitro evolution and crystallographic analysis of a peptide binding scFv antibody with low picomolar affinity
C Zahnd, S Spinelli, B Luginbuhl, P Amstutz… - Journal of Biological …, 2004 - ASBMB
We generated a single-chain antibody fragment (scFv) with a binding affinity of about 5 pM
to a short peptide by applying rigorous directed evolution. Starting from a high affinity
peptide binder, originally obtained by ribosome display from a murine library, we generated …
to a short peptide by applying rigorous directed evolution. Starting from a high affinity
peptide binder, originally obtained by ribosome display from a murine library, we generated …
