Detection of Methylobacterium species by 16S rRNA gene-targeted PCR
T Nishio, T Yoshikura, H Itoh - Applied and Environmental …, 1997 - Am Soc Microbiol
T Nishio, T Yoshikura, H Itoh
Applied and Environmental Microbiology, 1997•Am Soc MicrobiolWe designed PCR primers for specific amplification of the 16S rRNA genes of seven species
of the genus Methylobacterium. All of the pairwise species tested were successfully
differentiated by PCR detection with a combination of five primer sets, with the exception of
M. extorquens and M. rhodesianum. These primers did not cross-react with closely related
bacteria in the alpha subclass tested.
of the genus Methylobacterium. All of the pairwise species tested were successfully
differentiated by PCR detection with a combination of five primer sets, with the exception of
M. extorquens and M. rhodesianum. These primers did not cross-react with closely related
bacteria in the alpha subclass tested.
We designed PCR primers for specific amplification of the 16S rRNA genes of seven species of the genus Methylobacterium. All of the pairwise species tested were successfully differentiated by PCR detection with a combination of five primer sets, with the exception of M. extorquens and M. rhodesianum. These primers did not cross-react with closely related bacteria in the alpha subclass tested.
American Society for Microbiology