Neutral lipid storage disease (NLSD) is an autosomal recessive disorder characterized by
the presence of numerous lipid droplets in virtually all tissues examined. The increased
cellular triacylglycerol content results from defective recycling of triacylglycerol-derived
diacylglycerol to phospholipids (Igal, RA and RA Coleman. 1996. J. Biol. Chem. 271: 16644–
16651). In order to determine whether de novo glycerolipid synthesis is also altered in
NLSD, we compared the ability of normal human skin fibroblasts and fibroblasts from a …