Effect of freezing on the infectivity of Toxoplasma cysts to cats.
JP Dubey - 1974 - cabidigitallibrary.org
1974•cabidigitallibrary.org
Mice chronically infected with four strains of Toxoplasma gondii were killed and stored at-9
deg C and-20 deg C for 3, 4 and 6 hours and for 1 and 7 days. After thawing at 4 deg C or 22
deg C, the whole mice were fed to 8 toxoplasma-free cats. None of the cats shed oocysts or
developed antibody to T. gondii within 30 days. Homogenates of brain and skeletal muscles
of frozen mice were also noninfective to susceptible mice. Hearts from two sheep naturally
infected with Toxoplasma were infective to cats after storage at-20 deg C for 2 and 3 hours …
deg C and-20 deg C for 3, 4 and 6 hours and for 1 and 7 days. After thawing at 4 deg C or 22
deg C, the whole mice were fed to 8 toxoplasma-free cats. None of the cats shed oocysts or
developed antibody to T. gondii within 30 days. Homogenates of brain and skeletal muscles
of frozen mice were also noninfective to susceptible mice. Hearts from two sheep naturally
infected with Toxoplasma were infective to cats after storage at-20 deg C for 2 and 3 hours …
Abstract
Mice chronically infected with four strains of Toxoplasma gondii were killed and stored at -9 deg C and -20 deg C for 3, 4 and 6 hours and for 1 and 7 days. After thawing at 4 deg C or 22 deg C, the whole mice were fed to 8 toxoplasma-free cats. None of the cats shed oocysts or developed antibody to T. gondii within 30 days. Homogenates of brain and skeletal muscles of frozen mice were also noninfective to susceptible mice. Hearts from two sheep naturally infected with Toxoplasma were infective to cats after storage at -20 deg C for 2 and 3 hours but not after storage for 11 days at that temperature.
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