Quantification of total bacteria, enterobacteria and lactobacilli populations in pig digesta by real-time PCR
M Castillo, SM Martín-Orúe, EG Manzanilla… - Veterinary …, 2006 - Elsevier
Jejunum digesta samples were taken from weaning pigs in order to evaluate real-time PCR
(qPCR) as a method for quantifying pig gut bacteria. Total bacteria, lactobacilli and
enterobacteria were quantified by qPCR and the results were compared with those obtained
with traditional methods: 4′, 6-diamidino-2-phenylindole (DAPI staining) for total bacteria,
selective culture for lactobacilli and enterobacteria. Real-time PCR showed higher values in
terms of 16S rRNA gene copies than DAPI counts or CFU. Despite the differences, the …
(qPCR) as a method for quantifying pig gut bacteria. Total bacteria, lactobacilli and
enterobacteria were quantified by qPCR and the results were compared with those obtained
with traditional methods: 4′, 6-diamidino-2-phenylindole (DAPI staining) for total bacteria,
selective culture for lactobacilli and enterobacteria. Real-time PCR showed higher values in
terms of 16S rRNA gene copies than DAPI counts or CFU. Despite the differences, the …
