Analysis of bovine sexed sperm for IVF from sorting to the embryo
P Blondin, M Beaulieu, V Fournier, N Morin, L Crawford… - Theriogenology, 2009 - Elsevier
The objective of this study was to characterize bovine semen parameters and determine the
best IVF conditions to produce a maximal percentage of blastocysts. Four types of semen
were analyzed with CASA and flow cytometry: fresh and frozen non-sexed semen; fresh and
frozen sexed semen. Semen was obtained from four Holstein bulls and two ejaculates from
each bull were analyzed. Oocytes from slaughterhouse ovaries were matured and fertilized
in vitro with all types of semen (for sexed semen, 2, 5 or 10μg/mL heparin was added to the …
best IVF conditions to produce a maximal percentage of blastocysts. Four types of semen
were analyzed with CASA and flow cytometry: fresh and frozen non-sexed semen; fresh and
frozen sexed semen. Semen was obtained from four Holstein bulls and two ejaculates from
each bull were analyzed. Oocytes from slaughterhouse ovaries were matured and fertilized
in vitro with all types of semen (for sexed semen, 2, 5 or 10μg/mL heparin was added to the …
