Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries

MC Pieterse, KA Kappen, TAM Kruip, MAM Taverne - Theriogenology, 1988 - Elsevier
A technique for the repeated collection of bovine oocytes using transvaginal ultrasound
guided aspiration is described. Cows were used during their normal estrous cycle and after
stimulation of the ovaries with pregnant mare serum gonadotrophin (PMSG). The sedation of
the animals and the puncturing of follicles appears not to have traumatized the animals and
plasma progesterone measurements suggested that the cyclicity was not interrupted. A total
of 36 transvaginal aspiration procedures were performed, during which 54 oocytes were …