Recently FHWA restricted the use of certain sizes of seven-wire prestressing strand in the fabrication of highway bridge beams. The research reported is the result of the prestressing industry's interest in evaluating the effects of development length and transfer length on the behavior of AASHTO Type I beams. Additionally, variations in transfer and development length were investigated for different diameters of reinforcing and different wire manufacturers. Twenty-two full-scale AASHTO Type I beams were static-tested to failure; the results of these tests are reported. Factors that affect both transfer and development length are evaluated and discussed. The computed and measured moment capacities are compared, and reasons for variations are evaluated. Three distinct failure modes were observed, depending on the location of the load in relation to the development length of the prestressing strand. The relationship between failure modes and the ultimate moment capacity is evaluated.