Birth of offspring of pre-determined sex after artificial insemination of frozen–thawed, sex-sorted and re-frozen–thawed ram spermatozoa
SP De Graaf, G Evans, WMC Maxwell, DG Cran… - Theriogenology, 2007 - Elsevier
The fertility of ram spermatozoa cryopreserved prior to, and following, sex-sorting by flow
cytometry was assessed after insemination of mature Merino ewes at a synchronised estrus.
Ewes were inseminated with spermatozoa from three rams, split into four treatment groups:
50× 106 motile non-sorted, frozen–thawed (Control50), 15× 106 motile non-sorted, frozen–
thawed (Control15), 15× 106 motile sex-sorted, frozen–thawed (SF15) or 15× 106 motile
frozen–thawed, sex-sorted, re-frozen–thawed (FSF15) ram spermatozoa. Separation of …
cytometry was assessed after insemination of mature Merino ewes at a synchronised estrus.
Ewes were inseminated with spermatozoa from three rams, split into four treatment groups:
50× 106 motile non-sorted, frozen–thawed (Control50), 15× 106 motile non-sorted, frozen–
thawed (Control15), 15× 106 motile sex-sorted, frozen–thawed (SF15) or 15× 106 motile
frozen–thawed, sex-sorted, re-frozen–thawed (FSF15) ram spermatozoa. Separation of …
