Bovine in vitro fertilization with frozen-thawed semen
JJ Parrish, JL Susko-Parrish, ML Leibfried-Rutledge… - Theriogenology, 1986 - Elsevier
A procedure to obtain high and repeatable fertilization frequencies for bovine in vitro
fertilization (IVF) with frozen-thawed sperm was developed. IVF frequency of in vitro matured
oocytes was increased by a swimup sperm separation procedure (P= 0.01) or treatment of
sperm with the glycosaminoglycan heparin (P= 0.0001), but the two factors did not interact
(P= 0.23). Heparin was the most important factor in increasing IVF frequencies. The
fertilization frequency was not affected by the batch of oocytes used (P= 0.38), but bull …
fertilization (IVF) with frozen-thawed sperm was developed. IVF frequency of in vitro matured
oocytes was increased by a swimup sperm separation procedure (P= 0.01) or treatment of
sperm with the glycosaminoglycan heparin (P= 0.0001), but the two factors did not interact
(P= 0.23). Heparin was the most important factor in increasing IVF frequencies. The
fertilization frequency was not affected by the batch of oocytes used (P= 0.38), but bull …
