The purpose of this investigation was to clarify whether salivary cortisol secretion in dogs
had a circadian rhythm. Saliva sampling during a 24-hour period was performed in 4 non-
consecutive days. Eight adult beagle dogs (4 males and 4 females) were divided into 4
groups, and 2 dogs (1 male and 1 female) were used for each repetition. Saliva samples
were taken at 1 h intervals from 9: 00 am to 9: 00 am of the following day. Salivary cortisol
concentrations were determined using enzyme-linked immunosorbent assay. No circadian …

The purpose of this study was to determine if salivary chromogranin a secretion in dogs
exhibits a circadian rhythm. Saliva sampling was performed during three different sessions
occurring in three nonconsecutive 24-h periods. Sixteen healthy adult beagle dogs (8 males
and 8 females) were moved to a sampling room and housed individually in cages. Saliva
samples were obtained every 4 h from 12: 00 pm to 12: 00 pm the following day. In the
interest of habituation, saliva was obtained hourly from each dog 3 h before the experiment …