[HTML][HTML] Choosing a culture medium: making informed choices
JD Biggers, MC Summers - Fertility and sterility, 2008 - Elsevier
OBJECTIVE: To analyze critically the reasons justifying the choice of two-step protocols
requiring two media for the culture of human preimplantation embryos from the zygote to the
blastocyst. DESIGN: Literature review. RESULT (S): Two types of protocol are used for the
culture of human preimplantation embryos from the zygote to the blastocyst, using either one
medium (one-step protocol) or two media of different composition (two-step protocol). Two-
step protocols are the most widely used, largely because all but one of the commercially …
requiring two media for the culture of human preimplantation embryos from the zygote to the
blastocyst. DESIGN: Literature review. RESULT (S): Two types of protocol are used for the
culture of human preimplantation embryos from the zygote to the blastocyst, using either one
medium (one-step protocol) or two media of different composition (two-step protocol). Two-
step protocols are the most widely used, largely because all but one of the commercially …
