Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification
TL Nedambale, A Dinnyes, W Groen, JR Dobrinsky… - Theriogenology, 2004 - Elsevier
The objectives of this study were to identify an improved in vitro cell-free embryo culture
system and to compare post-warming development of in vitro produced (IVP) bovine
embryos following vitrification versus slow freezing. In Experiment 1, non-selected
presumptive zygotes were randomly allocated to four medium treatments without co-
culture:(1) SOF+ 5% FCS for 9 days;(2) KSOM+ 0.1% BSA for 4 days and then KSOM+ 1%
BSA to Day 9;(3) SOF+ 5% FCS for 4 days and then KSOM+ 1% BSA to Day 9; and (4) …
system and to compare post-warming development of in vitro produced (IVP) bovine
embryos following vitrification versus slow freezing. In Experiment 1, non-selected
presumptive zygotes were randomly allocated to four medium treatments without co-
culture:(1) SOF+ 5% FCS for 9 days;(2) KSOM+ 0.1% BSA for 4 days and then KSOM+ 1%
BSA to Day 9;(3) SOF+ 5% FCS for 4 days and then KSOM+ 1% BSA to Day 9; and (4) …
