Development of bovine IVF oocytes cultured in medium supplemented with a nitric oxide scavenger or inhibitor in a co-culture system
JM Lim, Y Mei, B Chen, RA Godke, W Hansel - Theriogenology, 1999 - Elsevier
Bovine IVF oocytes were cultured in modified bovine embryo culture medium (mBECM)
supplemented with either a nitric oxide (NO) scavenger, hemoglobin (Hb, 1 μg/mL) and/or a
NO synthesis inhibitor, Lω-nitro-L-arginine methyl ester (L-NAME, 1 or 1000 nM) in a
cumulusgranulosa cell co-culture system. In Experiment 1, a total of 1,675 cumulus-oocytes
complexes was collected for 7 mo and cultured to the blastocyst stage in mBECM with or
without Hb after IVM and IVF. There were significant (P< 0.0024) model effects of Hb …
supplemented with either a nitric oxide (NO) scavenger, hemoglobin (Hb, 1 μg/mL) and/or a
NO synthesis inhibitor, Lω-nitro-L-arginine methyl ester (L-NAME, 1 or 1000 nM) in a
cumulusgranulosa cell co-culture system. In Experiment 1, a total of 1,675 cumulus-oocytes
complexes was collected for 7 mo and cultured to the blastocyst stage in mBECM with or
without Hb after IVM and IVF. There were significant (P< 0.0024) model effects of Hb …
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