Development of bovine embryos after in vitro fertilization of oocytes with flow cytometrically sorted, stained and unsorted sperm from different bulls
M Zhang, KH Lu, GE Seidel Jr - Theriogenology, 2003 - Elsevier
The objective of this study was to determine the effect of staining bovine sperm, with or
without flow cytometry, on in vitro fertilization of bovine oocytes and blastocyst development.
Bovine oocytes (n= 4273) were fertilized with frozen–thawed sperm from three bulls that
was: stained with Hoechst 33342 and sorted (into X-or Y-chromosome-bearing sperm) with
flow cytometry; stained but not sorted; and not stained or sorted (Control). Oocytes, aspirated
from slaughterhouse ovaries, were matured in TCM199 (supplemented with 10% fetal calf …
without flow cytometry, on in vitro fertilization of bovine oocytes and blastocyst development.
Bovine oocytes (n= 4273) were fertilized with frozen–thawed sperm from three bulls that
was: stained with Hoechst 33342 and sorted (into X-or Y-chromosome-bearing sperm) with
flow cytometry; stained but not sorted; and not stained or sorted (Control). Oocytes, aspirated
from slaughterhouse ovaries, were matured in TCM199 (supplemented with 10% fetal calf …
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