Effect of delayed supplementation of fetal calf serum to culture medium on bovine embryo development in vitro and following transfer
JG Thompson, NW Allen, LT McGowan, ACS Bell… - Theriogenology, 1998 - Elsevier
Supplementation of synthetic oviduct fluid (SOF) medium plus amino acids and bovine
serum albumin (BSA) with either fetal calf serum (FCS) or charcoal-treated FCS (CT-FCS)
from Day 5 of development was investigated to determine if either in vitro or post-transfer
development was altered. Development to the compact morula stage or beyond was similar
for all 3 treatments. However, blastocyst development at Day 7 was accelerated when serum
was added to the medium (21.6, 40.1 and 39.4% blastocysts from cleaved embryos for BSA …
serum albumin (BSA) with either fetal calf serum (FCS) or charcoal-treated FCS (CT-FCS)
from Day 5 of development was investigated to determine if either in vitro or post-transfer
development was altered. Development to the compact morula stage or beyond was similar
for all 3 treatments. However, blastocyst development at Day 7 was accelerated when serum
was added to the medium (21.6, 40.1 and 39.4% blastocysts from cleaved embryos for BSA …
