Effects of heparin and sperm concentration on cleavage and blastocyst development rates of bovine oocytes inseminated with flow cytometrically-sorted sperm
KH Lu, GE Seidel Jr - Theriogenology, 2004 - Elsevier
The objective was to determine the optimal concentration of heparin for sperm capacitation,
as well as the optimal sperm concentration for in vitro fertilization using flow cytometrically-
sorted sperm from individual bulls. A total of 5327 bovine oocytes and sperm from four bulls
were examined. Oocytes from slaughterhouse ovaries were matured in TCM199 for 22–24h.
Flow cytometrically-sorted sperm as well as unsorted control sperm from the same bulls
were cryopreserved. For sperm from each of the four bulls, oocytes were inseminated in a …
as well as the optimal sperm concentration for in vitro fertilization using flow cytometrically-
sorted sperm from individual bulls. A total of 5327 bovine oocytes and sperm from four bulls
were examined. Oocytes from slaughterhouse ovaries were matured in TCM199 for 22–24h.
Flow cytometrically-sorted sperm as well as unsorted control sperm from the same bulls
were cryopreserved. For sperm from each of the four bulls, oocytes were inseminated in a …
