Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology
FA Ward, P Lonergan, BP Enright, MP Boland - Theriogenology, 2000 - Elsevier
In Experiment 1, different vacuum pressures (30, 50, 70 and 90 mm Hg) were used to
aspirate 4156 bovine follicles in vitro, to assess their effect on flow rate and the recovery,
morphology and blastocyst formation of the recovered oocytes Cumulus oocyte complexes
(COCs) were classified according to the morphology of the cumulus cells Data were
analyzed using Chi Square analysis Overall recovery rate declined as the aspiration
pressure increased above 50 mm Hg (P< 0 05) The recovery rate of Grade 1 oocytes …
aspirate 4156 bovine follicles in vitro, to assess their effect on flow rate and the recovery,
morphology and blastocyst formation of the recovered oocytes Cumulus oocyte complexes
(COCs) were classified according to the morphology of the cumulus cells Data were
analyzed using Chi Square analysis Overall recovery rate declined as the aspiration
pressure increased above 50 mm Hg (P< 0 05) The recovery rate of Grade 1 oocytes …
