High pressure flow cytometric sorting damages sperm
TK Suh, JL Schenk, GE Seidel Jr - Theriogenology, 2005 - Elsevier
Sexing sperm by high-speed flow cytometry subjects them to high pressure. The routine
operating pressure of the MoFlo® SX flow cytometer for sperm sorting for commercial
production has been 50pounds/square inch (psi), with a standard 70μm standard nozzle tip.
It was hypothesized that lowering the sorting pressure could reduce sperm damage.
Therefore, a series of experiments using semen from six bulls, sorted with three MoFlo® SX
sorters, was conducted to determine optimal pressure. An additional experiment was done …
operating pressure of the MoFlo® SX flow cytometer for sperm sorting for commercial
production has been 50pounds/square inch (psi), with a standard 70μm standard nozzle tip.
It was hypothesized that lowering the sorting pressure could reduce sperm damage.
Therefore, a series of experiments using semen from six bulls, sorted with three MoFlo® SX
sorters, was conducted to determine optimal pressure. An additional experiment was done …
