Improved cryopreservation of in vitro-produced bovine embryos using a chemically defined freezing medium
P Bruyère, A Baudot, C Guyader-Joly, P Guérin… - Theriogenology, 2012 - Elsevier
This study evaluates a new synthetic substitute (CRYO3, Ref. 5617, Stem Alpha, France) for
animal-based products in bovine embryo cryopreservation solutions. During the experiment,
fetal calf serum (FCS) and bovine serum albumin (BSA) were used as references. A
combination of a thermodynamic approach using differential scanning calorimetry and a
biological approach using in vitro-produced bovine embryo slow-freezing was used to
characterize cryopreservation solutions containing CRYO3, FCS and BSA. The CRYO3 and …
animal-based products in bovine embryo cryopreservation solutions. During the experiment,
fetal calf serum (FCS) and bovine serum albumin (BSA) were used as references. A
combination of a thermodynamic approach using differential scanning calorimetry and a
biological approach using in vitro-produced bovine embryo slow-freezing was used to
characterize cryopreservation solutions containing CRYO3, FCS and BSA. The CRYO3 and …
