In vitro production of bovine embryos in medium supplemented with a serum replacer: effects on blastocyst development, cryotolerance and survival to term
K Moore, CJ Rodriguez-Sallaberry, JM Kramer… - Theriogenology, 2007 - Elsevier
In this study, we evaluated a serum replacer (SR; Knockout SR®, Invitrogen) in our in vitro
culture systems. We hypothesized that SR would benefit bovine embryo development, since
SR supported survival of embryonic stem cells (which originate from embryos). Experiment 1
compared oocyte maturation with SR versus fetal bovine serum (FBS). Following fertilization,
blastocyst development was lower for oocytes matured with SR (21.5 versus 34.1, P< 0.05).
Experiment 2 evaluated SR for culturing embryos. Following fertilization, embryos were …
culture systems. We hypothesized that SR would benefit bovine embryo development, since
SR supported survival of embryonic stem cells (which originate from embryos). Experiment 1
compared oocyte maturation with SR versus fetal bovine serum (FBS). Following fertilization,
blastocyst development was lower for oocytes matured with SR (21.5 versus 34.1, P< 0.05).
Experiment 2 evaluated SR for culturing embryos. Following fertilization, embryos were …
