Interval of gonadotropin administration for in vitro embryo production from oocytes collected from Holstein calves between 2 and 6 months of age by repeated …

H Baldassarre, L Currin, L Michalovic, AM Bellefleur… - Theriogenology, 2018 - Elsevier
Abstract Laparoscopic Ovum Pick-Up (LOPU) in calves followed by in vitro embryo
production (IVEP) and transfer (ET) into adult recipients has great potential for accelerated
genetic gain through shortening of the generation interval. In this study, 11 Holstein calves
were subjected to up to six LOPU procedures between the ages of 2–6 months at 2–3 weeks
interval. In all cases, the animals received a CIDR 5 days prior to LOPU and were
gonadotropin-stimulated starting at 72 h before LOPU using one of three protocols that were …