Mapping and quantifying mammalian transcriptomes by RNA-Seq
A Mortazavi, BA Williams, K McCue, L Schaeffer… - Nature …, 2008 - nature.com
We have mapped and quantified mouse transcriptomes by deeply sequencing them and
recording how frequently each gene is represented in the sequence sample (RNA-Seq).
This provides a digital measure of the presence and prevalence of transcripts from known
and previously unknown genes. We report reference measurements composed of 41–52
million mapped 25-base-pair reads for poly (A)-selected RNA from adult mouse brain, liver
and skeletal muscle tissues. We used RNA standards to quantify transcript prevalence and …
recording how frequently each gene is represented in the sequence sample (RNA-Seq).
This provides a digital measure of the presence and prevalence of transcripts from known
and previously unknown genes. We report reference measurements composed of 41–52
million mapped 25-base-pair reads for poly (A)-selected RNA from adult mouse brain, liver
and skeletal muscle tissues. We used RNA standards to quantify transcript prevalence and …