Optimization of in vitro bovine embryo production: effect of duration of maturation, length of gamete co-incubation, sperm concentration and sire
F Ward, B Enright, D Rizos, M Boland, P Lonergan - Theriogenology, 2002 - Elsevier
The aim of these experiments was to investigate the effect of duration of IVM, duration of
gamete co-incubation, and of sperm dose on the development of bovine embryos in vitro. In
addition, the speed of sperm penetration of six bulls of known differing in vivo and in vitro
fertility was examined. In Experiment 1, following IVM for 16, 20, 24, 28 or 32h, cumulus
oocyte complexes (COCs) were inseminated with 1× 106spermatozoa/ml. After 24h co-
incubation, presumptive zygotes were denuded and placed in droplets of synthetic oviduct …
gamete co-incubation, and of sperm dose on the development of bovine embryos in vitro. In
addition, the speed of sperm penetration of six bulls of known differing in vivo and in vitro
fertility was examined. In Experiment 1, following IVM for 16, 20, 24, 28 or 32h, cumulus
oocyte complexes (COCs) were inseminated with 1× 106spermatozoa/ml. After 24h co-
incubation, presumptive zygotes were denuded and placed in droplets of synthetic oviduct …
