Supplying and maintaining appropriate culture conditions is critical to minimize stress
imposed upon gametes and embryos and to optimize the in-vitro environment. One
parameter that requires close scrutiny in this endeavour is pH. Though embryos have a
limited ability to regulate their internal pH (pHi), oocytes lack robust mechanisms. Thus,
careful attention to external pH (pHe) of culture media is imperative in IVF. Ability to
withstand deviations in hydrogen ion concentration varies depending on culture conditions …